Regenerative Surface Plasmon Resonance (SPR) biosensor: Real-time measurement of fibrinogen in undiluted human serum using the competitive adsorption of proteins

Ran Wang, Arad Lajevardi-Khosh, Seokheun Choi, Junseok Chae

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Epidemiological studies suggest that elevated plasma fibrinogen levels are associated with an increased risk of cardiovascular disorders. Normal fibrinogen level is in the range of 1.5-4.5. mg/mL, depending upon both genetic (intrinsic) and environmental (extrinsic) factors. An increase of 0.25 mg/mL from the normal level can often be correlated with a high risk of cardiovascular disease. Thus, it is useful to monitor fibrinogen level in serum of a patient for clinical diagnosis. We report a regenerative biosensor that measures real-time fibrinogen levels in undiluted serum. The biosensor uses Surface Plasmon Resonance (SPR), highly sensitive optical technique. The biosensor does not use bio-receptors (i.e., antibodies, enzymes, DNA, etc.) unlike conventional biosensors, and deploys the nature of competitive adsorption of proteins to achieve selective detection of fibrinogen. We measured fibrinogen-spiked serum samples with a concentration of 1.5-4.5. mg/mL, and repeated six measurement trials to obtain statistical distribution of the measurements using the regeneration method of the sensing surface. The SPR biosensor has a sensitivity of 42 mDeg/(mg/mL) for a fibrinogen concentration in the range of 0.5-2.5. mg/mL, whereas it was hard to correlate the measurements to the spiked-fibrinogen samples of above 2.5. mg/mL.

Original languageEnglish (US)
Pages (from-to)304-307
Number of pages4
JournalBiosensors and Bioelectronics
Volume28
Issue number1
DOIs
StatePublished - Oct 15 2011

Keywords

  • Fibrinogen
  • Human serum
  • Regenerative biosensor
  • Surface Plasmon Resonance (SPR)
  • Vroman effect

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Fingerprint Dive into the research topics of 'Regenerative Surface Plasmon Resonance (SPR) biosensor: Real-time measurement of fibrinogen in undiluted human serum using the competitive adsorption of proteins'. Together they form a unique fingerprint.

  • Cite this