TY - JOUR
T1 - Regenerative Surface Plasmon Resonance (SPR) biosensor
T2 - Real-time measurement of fibrinogen in undiluted human serum using the competitive adsorption of proteins
AU - Wang, Ran
AU - Lajevardi-Khosh, Arad
AU - Choi, Seokheun
AU - Chae, Junseok
PY - 2011/10/15
Y1 - 2011/10/15
N2 - Epidemiological studies suggest that elevated plasma fibrinogen levels are associated with an increased risk of cardiovascular disorders. Normal fibrinogen level is in the range of 1.5-4.5. mg/mL, depending upon both genetic (intrinsic) and environmental (extrinsic) factors. An increase of 0.25 mg/mL from the normal level can often be correlated with a high risk of cardiovascular disease. Thus, it is useful to monitor fibrinogen level in serum of a patient for clinical diagnosis. We report a regenerative biosensor that measures real-time fibrinogen levels in undiluted serum. The biosensor uses Surface Plasmon Resonance (SPR), highly sensitive optical technique. The biosensor does not use bio-receptors (i.e., antibodies, enzymes, DNA, etc.) unlike conventional biosensors, and deploys the nature of competitive adsorption of proteins to achieve selective detection of fibrinogen. We measured fibrinogen-spiked serum samples with a concentration of 1.5-4.5. mg/mL, and repeated six measurement trials to obtain statistical distribution of the measurements using the regeneration method of the sensing surface. The SPR biosensor has a sensitivity of 42 mDeg/(mg/mL) for a fibrinogen concentration in the range of 0.5-2.5. mg/mL, whereas it was hard to correlate the measurements to the spiked-fibrinogen samples of above 2.5. mg/mL.
AB - Epidemiological studies suggest that elevated plasma fibrinogen levels are associated with an increased risk of cardiovascular disorders. Normal fibrinogen level is in the range of 1.5-4.5. mg/mL, depending upon both genetic (intrinsic) and environmental (extrinsic) factors. An increase of 0.25 mg/mL from the normal level can often be correlated with a high risk of cardiovascular disease. Thus, it is useful to monitor fibrinogen level in serum of a patient for clinical diagnosis. We report a regenerative biosensor that measures real-time fibrinogen levels in undiluted serum. The biosensor uses Surface Plasmon Resonance (SPR), highly sensitive optical technique. The biosensor does not use bio-receptors (i.e., antibodies, enzymes, DNA, etc.) unlike conventional biosensors, and deploys the nature of competitive adsorption of proteins to achieve selective detection of fibrinogen. We measured fibrinogen-spiked serum samples with a concentration of 1.5-4.5. mg/mL, and repeated six measurement trials to obtain statistical distribution of the measurements using the regeneration method of the sensing surface. The SPR biosensor has a sensitivity of 42 mDeg/(mg/mL) for a fibrinogen concentration in the range of 0.5-2.5. mg/mL, whereas it was hard to correlate the measurements to the spiked-fibrinogen samples of above 2.5. mg/mL.
KW - Fibrinogen
KW - Human serum
KW - Regenerative biosensor
KW - Surface Plasmon Resonance (SPR)
KW - Vroman effect
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U2 - 10.1016/j.bios.2011.07.036
DO - 10.1016/j.bios.2011.07.036
M3 - Article
C2 - 21820893
AN - SCOPUS:80052391016
SN - 0956-5663
VL - 28
SP - 304
EP - 307
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -