Recombinant human granulocyte/macrophage colony-stimulating factor enhances monocyte cytotoxicity and secretion of tumor necrosis factor α and interferon in cancer patients

E. J. Wing, Dewey Magee, T. L. Whiteside, S. S. Kaplan, R. K. Shadduck

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Abstract

The colony-stimulating factors (CSFs) promote the proliferation and differentiation of hematopoietic precursors and more recently have been shown to amplify the functions of mature phagocytes in vitro. In this study recombinant human granulocyte/macrophage colony-stimulating factor (rGM-CSF) was administered to cancer patients to determine whether the cytotoxic and secretory activity of their blood monocytes could be enhanced. Patients with refractory neoplastic disease were treated with rGM-CSF either as a single bolus or as a constant infusion for 14 days at either 100 or 500 μg/m2 per day. As has been reported by others, the number of peripheral blood monocytes and granulocytes rose markedly in a dose-response fashion during infusion with rGM-CSF. The functional capacity of monocytes was increased by rGM-CSF, since the cytotoxicity of monocytes against antibody-coated xenogeneic cells was increased during the constant infusion compared to baseline. In addition, monocytes harvested during the constant infusion and stimulated with lipopolysaccharide (LPS) in vitro secreted increased quantities of tumor necrosis factor α (TNF-α) and interferon (IFN). These data indicate that rGM-CSF can enhance both the number and the function of peripheral blood monocytes in vivo.

Original languageEnglish (US)
Pages (from-to)643-646
Number of pages4
JournalBlood
Volume73
Issue number3
StatePublished - 1989
Externally publishedYes

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Granulocyte-Macrophage Colony-Stimulating Factor
Cytotoxicity
Interferons
Monocytes
Tumor Necrosis Factor-alpha
Blood
Neoplasms
Colony-Stimulating Factors
Heterophile Antibodies
Refractory materials
Lipopolysaccharides
Phagocytes
Granulocytes
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Recombinant human granulocyte/macrophage colony-stimulating factor enhances monocyte cytotoxicity and secretion of tumor necrosis factor α and interferon in cancer patients. / Wing, E. J.; Magee, Dewey; Whiteside, T. L.; Kaplan, S. S.; Shadduck, R. K.

In: Blood, Vol. 73, No. 3, 1989, p. 643-646.

Research output: Contribution to journalArticle

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AU - Kaplan, S. S.

AU - Shadduck, R. K.

PY - 1989

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AB - The colony-stimulating factors (CSFs) promote the proliferation and differentiation of hematopoietic precursors and more recently have been shown to amplify the functions of mature phagocytes in vitro. In this study recombinant human granulocyte/macrophage colony-stimulating factor (rGM-CSF) was administered to cancer patients to determine whether the cytotoxic and secretory activity of their blood monocytes could be enhanced. Patients with refractory neoplastic disease were treated with rGM-CSF either as a single bolus or as a constant infusion for 14 days at either 100 or 500 μg/m2 per day. As has been reported by others, the number of peripheral blood monocytes and granulocytes rose markedly in a dose-response fashion during infusion with rGM-CSF. The functional capacity of monocytes was increased by rGM-CSF, since the cytotoxicity of monocytes against antibody-coated xenogeneic cells was increased during the constant infusion compared to baseline. In addition, monocytes harvested during the constant infusion and stimulated with lipopolysaccharide (LPS) in vitro secreted increased quantities of tumor necrosis factor α (TNF-α) and interferon (IFN). These data indicate that rGM-CSF can enhance both the number and the function of peripheral blood monocytes in vivo.

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