Receptor interactive protein kinase 3 promotes cisplatin-triggered necrosis in apoptosis-resistant esophageal squamous cell carcinoma cells

Yang Xu, Zhengwei Lin, Nan Zhao, Lanping Zhou, Fang Liu, Zbigniew Cichacz, Lin Zhang, Qimin Zhan, Xiaohang Zhao

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cisplatin-based chemotherapy is currently the standard treatment for locally advanced esophageal cancer. Cisplatin has been shown to induce both apoptosis and necrosis in cancer cells, but the mechanism by which programmed necrosis is induced remains unknown. In this study, we provide evidence that cisplatin induces necrotic cell death in apoptosisresistant esophageal cancer cells. This cell death is dependent on RIPK3 and on necrosome formation via autocrine production of TNFα. More importantly, we demonstrate that RIPK3 is necessary for cisplatin-induced killing of esophageal cancer cells because inhibition of RIPK1 activity by necrostatin or knockdown of RIPK3 significantly attenuates necrosis and leads to cisplatin resistance. Moreover, microarray analysis confirmed an anti-apoptotic molecular expression pattern in esophageal cancer cells in response to cisplatin. Taken together, our data indicate that RIPK3 and autocrine production of TNFα contribute to cisplatin sensitivity by initiating necrosis when the apoptotic pathway is suppressed or absent in esophageal cancer cells. These data provide new insight into the molecular mechanisms underlying cisplatin-induced necrosis and suggest that RIPK3 is a potential marker for predicting cisplatin sensitivity in apoptosis-resistant and advanced esophageal cancer.

Original languageEnglish (US)
Article numbere100127
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 24 2014

Fingerprint

cisplatin
squamous cell carcinoma
protein kinases
Protein Kinases
Cisplatin
esophageal neoplasms
necrosis
Necrosis
apoptosis
Cells
Esophageal Neoplasms
Apoptosis
receptors
cells
Cell death
cell death
Cell Death
Esophageal Squamous Cell Carcinoma
Epithelial Cells
Chemotherapy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Receptor interactive protein kinase 3 promotes cisplatin-triggered necrosis in apoptosis-resistant esophageal squamous cell carcinoma cells. / Xu, Yang; Lin, Zhengwei; Zhao, Nan; Zhou, Lanping; Liu, Fang; Cichacz, Zbigniew; Zhang, Lin; Zhan, Qimin; Zhao, Xiaohang.

In: PLoS One, Vol. 9, No. 6, e100127, 24.06.2014.

Research output: Contribution to journalArticle

Xu, Yang ; Lin, Zhengwei ; Zhao, Nan ; Zhou, Lanping ; Liu, Fang ; Cichacz, Zbigniew ; Zhang, Lin ; Zhan, Qimin ; Zhao, Xiaohang. / Receptor interactive protein kinase 3 promotes cisplatin-triggered necrosis in apoptosis-resistant esophageal squamous cell carcinoma cells. In: PLoS One. 2014 ; Vol. 9, No. 6.
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