21 Citations (Scopus)

Abstract

Phosphorylation is a post-translational modification that is involved in many basic cellular processes and diseases, but is difficult to detect in real time with existing technologies. A label-free detection of phosphorylation is reported in real time with self-assembled nano-oscillators. Each nano-oscillator consists of a gold nanoparticle tethered to a gold surface with a molecular linker. When the nanoparticle is charged, the nano-oscillator can be driven into oscillation with an electric field and detected with a plasmonic imaging approach. The nano-oscillators measure charge change associated with phosphorylation of peptides attached onto a single nanoparticle, allowing us to study the dynamic process of phosphorylation in real time without antibodies down to a few molecules, from which Michaelis and catalytic rate constants are determined.

Original languageEnglish (US)
Pages (from-to)2538-2542
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number8
DOIs
StatePublished - Feb 16 2015

Fingerprint

Phosphorylation
Kinetics
Monitoring
Nanoparticles
Gold
Antibodies
Peptides
Labels
Rate constants
Electric fields
Imaging techniques
Molecules

Keywords

  • Charge-based detection
  • Nano-oscillators
  • Phosphorylation
  • Plasmonic imaging
  • Real-time imaging

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Real-time monitoring of phosphorylation kinetics with self-assembled nano-oscillators. / Fang, Yimin; Chen, Shan; Wang, Wei; Shan, Xiaonan; Tao, Nongjian.

In: Angewandte Chemie - International Edition, Vol. 54, No. 8, 16.02.2015, p. 2538-2542.

Research output: Contribution to journalArticle

Fang, Yimin ; Chen, Shan ; Wang, Wei ; Shan, Xiaonan ; Tao, Nongjian. / Real-time monitoring of phosphorylation kinetics with self-assembled nano-oscillators. In: Angewandte Chemie - International Edition. 2015 ; Vol. 54, No. 8. pp. 2538-2542.
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