Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa

Kaewta Rattanapisit, Anchalee Srijangwad, Taksina Chuanasa, Suchada Sukrong, Angkana Tantituvanont, Hugh Mason, Dachrit Nilubol, Waranyoo Phoolcharoen

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration, weight loss, and high mortality rate in neonatal piglets. Porcine epidemic diarrhea (PED) has been reported in Europe, America, and Asia including Thailand. The disease causes substantial losses to the swine industry in many countries. Presently, there is no effective PEDV vaccine available. In this study, we developed a plant-produced monoclonal antibody (mAb) 2C10 as a prophylactic candidate to prevent the PEDV infection. Recently, plant expression systems have gained interest as an alternative for the production of antibodies because of many advantages, such as low production cost, lack of human and animal pathogen, large scalability, etc. The 2C10 mAb was transiently expressed in Nicotiana benthamiana and lettuce using geminiviral vector. After purification by protein A affinity chromatography, the antibody was tested for the binding and neutralizing activity against PEDV. Our result showed that the plant produced 2C10 mAb can bind to the virus and also inhibit PEDV infection in vitro. These results show excellent potential for a plant-expressed 2C10 as a PEDV prophylaxis and a diagnostic for PEDV infection.

Original languageEnglish (US)
Pages (from-to)1412-1419
Number of pages8
JournalPlanta Medica
Volume83
Issue number18
DOIs
StatePublished - Dec 1 2017

Keywords

  • 2C10 monoclonal antibody (mAb)
  • Asteraceae
  • Lactuca sativa
  • Nicotiana benthamiana
  • Solanaceae
  • molecular farming
  • porcine epidemic diarrhea virus (PEDV)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa'. Together they form a unique fingerprint.

  • Cite this

    Rattanapisit, K., Srijangwad, A., Chuanasa, T., Sukrong, S., Tantituvanont, A., Mason, H., Nilubol, D., & Phoolcharoen, W. (2017). Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa. Planta Medica, 83(18), 1412-1419. https://doi.org/10.1055/s-0043-112344