Rapid transient expression of cholera toxin B subunit (CTB) in Nicotiana benthamiana

Kaewta Rattanapisit, Seong Hee Bhoo, Tae Ryong Hahn, Hugh Mason, Waranyoo Phoolcharoen

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Cholera toxin B subunit (CTB) has the potential to be an effective adjuvant for mucosal vaccines because of its ability to increase antigen uptake and presentation by antigen-presenting cells through GM1-ganglioside binding. CTB has been produced using different recombinant protein expression systems. This study used the geminiviral replicon system to transiently express CTB in Nicotiana benthamiana. The plant-optimized CTB gene was cloned into a geminiviral vector and infiltrated into N. benthamiana leaves. The highest CTB protein level was observed on day 4 with approximately 4 μg/g fresh weight. The Western blot analysis using anti-CTB suggests assembly of CTB into oligomers. Based on the GM1-ELISA results, this CTB transiently expressed in plants showed biological activity for binding the intestinal epithelial cell membrane glycolipid receptor, GM1-glanglioside, which implies its potential as an adjuvant for mucosal vaccines.

Original languageEnglish (US)
Pages (from-to)107-113
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume49
Issue number2
DOIs
StatePublished - Jan 1 2013

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Keywords

  • Cholera toxin B subunit (CTB)
  • Geminiviral replicon system
  • Nicotiana benthamiana
  • Transient expression

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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