Rapid RFLP screening procedure identifies new polymorphisms at albumin and alcohol dehydrogenase loci

Jeffrey C. Murray; Rita Shiang; Leon R. Carlock; Moyra Smith; Kenneth H. Buetow

doi:10.1007/BF00283622

Rapid RFLP screening procedure identifies new polymorphisms at albumin and alcohol dehydrogenase loci

Jeffrey C. Murray, Rita Shiang, Leon R. Carlock, Moyra Smith, Kenneth H. Buetow

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A rapid screening procedure for restriction fragment length polymorphisms (RFLPs) is reported. DNA from ten individuals is pooled and compared to DNA isolated from a cell line containing a single chromosome 4. This single chromosome-containing line is an obligate hemizygote for chromosome 4 RFLPs so that only one band corresponding to a single allele will appear on a Southern blot. In the pooled DNA sample lane bands corresponding to both alleles will be seen. The technique allows for efficient detection of RFLPs with easier use of large numbers of enzymes. It provides estimates of allele frequencies and disequilibrium. New RFLPs for albumin and alcohol dehydrogenase detected with this technique are described.

Original language	English (US)
Pages (from-to)	274-277
Number of pages	4
Journal	Human Genetics
Volume	76
Issue number	3
DOIs	https://doi.org/10.1007/BF00283622
State	Published - Jul 1987
Externally published	Yes

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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title = "Rapid RFLP screening procedure identifies new polymorphisms at albumin and alcohol dehydrogenase loci",

abstract = "A rapid screening procedure for restriction fragment length polymorphisms (RFLPs) is reported. DNA from ten individuals is pooled and compared to DNA isolated from a cell line containing a single chromosome 4. This single chromosome-containing line is an obligate hemizygote for chromosome 4 RFLPs so that only one band corresponding to a single allele will appear on a Southern blot. In the pooled DNA sample lane bands corresponding to both alleles will be seen. The technique allows for efficient detection of RFLPs with easier use of large numbers of enzymes. It provides estimates of allele frequencies and disequilibrium. New RFLPs for albumin and alcohol dehydrogenase detected with this technique are described.",

author = "Murray, {Jeffrey C.} and Rita Shiang and Carlock, {Leon R.} and Moyra Smith and Buetow, {Kenneth H.}",

year = "1987",

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T1 - Rapid RFLP screening procedure identifies new polymorphisms at albumin and alcohol dehydrogenase loci

AU - Murray, Jeffrey C.

AU - Shiang, Rita

AU - Carlock, Leon R.

AU - Smith, Moyra

AU - Buetow, Kenneth H.

PY - 1987/7

Y1 - 1987/7

N2 - A rapid screening procedure for restriction fragment length polymorphisms (RFLPs) is reported. DNA from ten individuals is pooled and compared to DNA isolated from a cell line containing a single chromosome 4. This single chromosome-containing line is an obligate hemizygote for chromosome 4 RFLPs so that only one band corresponding to a single allele will appear on a Southern blot. In the pooled DNA sample lane bands corresponding to both alleles will be seen. The technique allows for efficient detection of RFLPs with easier use of large numbers of enzymes. It provides estimates of allele frequencies and disequilibrium. New RFLPs for albumin and alcohol dehydrogenase detected with this technique are described.

AB - A rapid screening procedure for restriction fragment length polymorphisms (RFLPs) is reported. DNA from ten individuals is pooled and compared to DNA isolated from a cell line containing a single chromosome 4. This single chromosome-containing line is an obligate hemizygote for chromosome 4 RFLPs so that only one band corresponding to a single allele will appear on a Southern blot. In the pooled DNA sample lane bands corresponding to both alleles will be seen. The technique allows for efficient detection of RFLPs with easier use of large numbers of enzymes. It provides estimates of allele frequencies and disequilibrium. New RFLPs for albumin and alcohol dehydrogenase detected with this technique are described.

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Rapid RFLP screening procedure identifies new polymorphisms at albumin and alcohol dehydrogenase loci

Abstract

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