TY - JOUR
T1 - Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad
AU - Hartmann, Erica M.
AU - Badalamenti, Jonathan P.
AU - Krajmalnik-Brown, Rosa
AU - Halden, Rolf
PY - 2012/6
Y1 - 2012/6
N2 - We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenaseencoding genes.
AB - We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenaseencoding genes.
UR - http://www.scopus.com/inward/record.url?scp=84864087678&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864087678&partnerID=8YFLogxK
U2 - 10.1128/AEM.00715-12
DO - 10.1128/AEM.00715-12
M3 - Article
C2 - 22492441
AN - SCOPUS:84864087678
SN - 0099-2240
VL - 78
SP - 4493
EP - 4496
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 12
ER -