Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad

Erica M. Hartmann, Jonathan P. Badalamenti, Rosa Krajmalnik-Brown, Rolf Halden

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenaseencoding genes.

Original languageEnglish (US)
Pages (from-to)4493-4496
Number of pages4
JournalApplied and Environmental Microbiology
Volume78
Issue number12
DOIs
StatePublished - Jun 2012

Fingerprint

Sphingomonas wittichii
biodegradation
plasmids
quantitative polymerase chain reaction
Polymerase Chain Reaction
gene
Chemical Water Pollutants
Sphingomonas
landfill leachates
Genes
dioxins
genes
dioxin
degradation
monitoring
Growth
methodology
sampling
method

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad. / Hartmann, Erica M.; Badalamenti, Jonathan P.; Krajmalnik-Brown, Rosa; Halden, Rolf.

In: Applied and Environmental Microbiology, Vol. 78, No. 12, 06.2012, p. 4493-4496.

Research output: Contribution to journalArticle

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