Abstract
Combining immunocytochemistry with in situ hybridization of Alzheimer disease (AD) hippocampus demonstrated a 50% reduction in grain density for synaptophysin message over CA1 pyramidal neurons containing neurofibrillary tangles (NFT) relative to near neighbor NFT-free neurons. This decrease was not global, but was selective since message grain density for the lysosomal protein, cathepsin D, increased 33% in these neurons (relative to NFT-free neurons). Poly A+ message grain density decreased by 25% in NFT neurons. Percent of the cell body containing NFT correlated -0.35 (p < 0.0001) with grain density for synaptophysin message. These data verify the concept of altered profiles of gene expression as a function of disease state within single cells and suggest that events associated with NFT formation may lead to altered expression of synaptic messages.
Original language | English (US) |
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Pages (from-to) | 275-287 |
Number of pages | 13 |
Journal | Journal of Neuropathology and Experimental Neurology |
Volume | 58 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1999 |
Externally published | Yes |
Keywords
- CA1
- Cathepsin D
- Message
- NFT
- NFT-free
- Quantitation
- Synaptophysin
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Neurology
- Clinical Neurology
- Cellular and Molecular Neuroscience