Quantitative decrease in synaptophysin message expression and increase in cathepsin D message expression in Alzheimer disease neurons containing neurofibrillary tangles

Linda M. Callahan, William A. Vaules, Paul D. Coleman

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Combining immunocytochemistry with in situ hybridization of Alzheimer disease (AD) hippocampus demonstrated a 50% reduction in grain density for synaptophysin message over CA1 pyramidal neurons containing neurofibrillary tangles (NFT) relative to near neighbor NFT-free neurons. This decrease was not global, but was selective since message grain density for the lysosomal protein, cathepsin D, increased 33% in these neurons (relative to NFT-free neurons). Poly A+ message grain density decreased by 25% in NFT neurons. Percent of the cell body containing NFT correlated -0.35 (p < 0.0001) with grain density for synaptophysin message. These data verify the concept of altered profiles of gene expression as a function of disease state within single cells and suggest that events associated with NFT formation may lead to altered expression of synaptic messages.

Original languageEnglish (US)
Pages (from-to)275-287
Number of pages13
JournalJournal of Neuropathology and Experimental Neurology
Volume58
Issue number3
DOIs
StatePublished - Mar 1999
Externally publishedYes

Keywords

  • CA1
  • Cathepsin D
  • Message
  • NFT
  • NFT-free
  • Quantitation
  • Synaptophysin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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