Quantification of cytokines involved in wound healing using surface plasmon resonance

Tina M. Battaglia, Jean Francois Masson, Michael Sierks, Stephen P. Beaudoin, Joseph Rogers, Kevin N. Foster, G. Allen Holloway, Karl S. Booksh

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Sensing of three cytokines related to chronic wound healing, interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), with detection limits at or below 1 ng/mL in buffered saline solution and spiked cell culture medium (CCM) has been achieved. Fiber-optic surface plasmon resonance (SPR) sensors are coated with an antibody binding layer and antibodies specific to the cytokine of interest are covalently attached to this layer. To achieve such detection limits in a complex medium such as CCM, total protein content of 4 mg/mL, the use of a novel N-hydroxysuccinimide ester of 16-mercapto-hexadecanoic acid (NHS-MHA) is necessary. A comparison of the detection limits for IL-6 using currently widely used CM-dextran and NHS-MHA shows an improvement by a factor of 3 using NHS-MHA. The detection limits for the monitoring of cytokines in spiked saline solutions and CCM were similar for TNF-α and slightly higher for IL-1 and IL-6. The detection of each cytokine in the presence of interfering agents resulted in concentration prediction well within the error of calibration. The SPR sensors are stable in CCM after 20 min of pretreatment in CCM, minimizing the reliance on a reference sensor to quantify the cytokines in complex media. This technique enables a major advancement in the field of real-time monitoring of biologically relevant molecules in complex biological fluids.

Original languageEnglish (US)
Pages (from-to)7016-7023
Number of pages8
JournalAnalytical Chemistry
Volume77
Issue number21
DOIs
StatePublished - Nov 1 2005

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Surface plasmon resonance
Cell culture
Culture Media
Palmitic Acid
Cytokines
Interleukin-6
Esters
Interleukin-1
Sodium Chloride
Sensors
Tumor Necrosis Factor-alpha
Antibodies
Monitoring
Dextrans
Fiber optics
Calibration
Molecules
Fluids
N-hydroxysuccinimide
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Battaglia, T. M., Masson, J. F., Sierks, M., Beaudoin, S. P., Rogers, J., Foster, K. N., ... Booksh, K. S. (2005). Quantification of cytokines involved in wound healing using surface plasmon resonance. Analytical Chemistry, 77(21), 7016-7023. https://doi.org/10.1021/ac050568w

Quantification of cytokines involved in wound healing using surface plasmon resonance. / Battaglia, Tina M.; Masson, Jean Francois; Sierks, Michael; Beaudoin, Stephen P.; Rogers, Joseph; Foster, Kevin N.; Holloway, G. Allen; Booksh, Karl S.

In: Analytical Chemistry, Vol. 77, No. 21, 01.11.2005, p. 7016-7023.

Research output: Contribution to journalArticle

Battaglia, TM, Masson, JF, Sierks, M, Beaudoin, SP, Rogers, J, Foster, KN, Holloway, GA & Booksh, KS 2005, 'Quantification of cytokines involved in wound healing using surface plasmon resonance', Analytical Chemistry, vol. 77, no. 21, pp. 7016-7023. https://doi.org/10.1021/ac050568w
Battaglia, Tina M. ; Masson, Jean Francois ; Sierks, Michael ; Beaudoin, Stephen P. ; Rogers, Joseph ; Foster, Kevin N. ; Holloway, G. Allen ; Booksh, Karl S. / Quantification of cytokines involved in wound healing using surface plasmon resonance. In: Analytical Chemistry. 2005 ; Vol. 77, No. 21. pp. 7016-7023.
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