PYK2 as a Mediator of Endothelin-1/Gα11 Signaling to GLUT4 Glucose Transporters

Jin Park, Avirup Bose, John Leszyk, Michael P. Czech

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Endothelin-1 (ET-1) signaling through Gαq/11 stimulates translocation of intracellular GLUT4 glucose transporters to the plasma membrane of 3T3-L1 adipocytes by an unknown mechanism that requires protein tyrosine phosphorylation and ADP-ribosylation factor 6 (ARF6) but is independent of phosphatidylinositol 3 (PI3)-kinase. In contrast, insulin action on this process requires PI3-kinase but not ARF6. Here we report the identification of two proteins selectively tyrosine-phosphorylated in response to ET-1 but not insulin: the Ca2+-activated tyrosine kinase PYK2 and its physiological substrate, the adhesion scaffold protein paxillin. Endogenous paxillin as well as expressed Myc-tagged PYK2 or a Myc-tagged kinase-deficient PYK2 protein were acutely directed to F-actin-rich adhesion sites from the adipocyte cytoplasm in response to ET-1 but not insulin. CADTK-related non-kinase (CRNK) is a dominant negative form of PYK2 containing the C-terminal portion of the protein, which binds paxillin but lacks the PYK2 autophosphorylation site (Tyr402). CRNK expression in 3T3-L1 adipocytes inhibited ET-1-mediated F-actin polymerization and translocation of Myc-tagged GLUT4-enhanced green fluorescent protein (EGFP) to the plasma membrane without disrupting insulin action on these processes. These data reveal the tyrosine kinase PYK2 as a required signaling element in the regulation of GLUT4 recycling in 3T3-L1 adipocytes by ET-1, whereas insulin signaling is directed through a different pathway.

Original languageEnglish (US)
Pages (from-to)47751-47754
Number of pages4
JournalJournal of Biological Chemistry
Volume276
Issue number51
DOIs
StatePublished - Dec 21 2001
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Endothelin-1
Paxillin
Adipocytes
Insulin
Phosphatidylinositol 3-Kinase
Cell membranes
Proteins
Protein-Tyrosine Kinases
Tyrosine
Actins
Adhesion
Cell Membrane
Phosphorylation
Recycling
Scaffolds
Polymerization
Cytoplasm
Phosphotransferases
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

PYK2 as a Mediator of Endothelin-1/Gα11 Signaling to GLUT4 Glucose Transporters. / Park, Jin; Bose, Avirup; Leszyk, John; Czech, Michael P.

In: Journal of Biological Chemistry, Vol. 276, No. 51, 21.12.2001, p. 47751-47754.

Research output: Contribution to journalArticle

Park, Jin ; Bose, Avirup ; Leszyk, John ; Czech, Michael P. / PYK2 as a Mediator of Endothelin-1/Gα11 Signaling to GLUT4 Glucose Transporters. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 51. pp. 47751-47754.
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