Purification and structural study of the voltage-sensor domain of the human KCNQ1 potassium ion channel

Dungeng Peng, Ji Hun Kim, Brett M. Kroncke, Cheryl L. Law, Yan Xia, Kristin D. Droege, Wade Van Horn, Carlos G. Vanoye, Charles R. Sanders

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

KCNQ1 (also known as KV7.1 or KVLQT1) is a voltage-gated potassium channel modulated by members of the KCNE protein family. Among multiple functions, KCNQ1 plays a critical role in the cardiac action potential. This channel is also subject to inherited mutations that cause certain cardiac arrhythmias and deafness. In this study, we report the overexpression, purification, and preliminary structural characterization of the voltage-sensor domain (VSD) of human KCNQ1 (Q1-VSD). Q1-VSD was expressed in Escherichia coli and purified into lyso-palmitoylphosphatidylglycerol micelles, conditions under which this tetraspan membrane protein yields excellent nuclear magnetic resonance (NMR) spectra. NMR studies reveal that Q1-VSD shares a common overall topology with other channel VSDs, with an S0 helix followed by transmembrane helices S1-S4. The exact sequential locations of the helical spans do, however, show significant variations from those of the homologous segments of previously characterized VSDs. The S4 segment of Q1-VSD was seen to be α-helical (with no 310 component) and underwent rapid backbone amide H-D exchange over most of its length. These results lay the foundation for more advanced structural studies and can be used to generate testable hypotheses for future structure-function experiments.

Original languageEnglish (US)
Pages (from-to)2032-2042
Number of pages11
JournalBiochemistry
Volume53
Issue number12
DOIs
StatePublished - Apr 1 2014
Externally publishedYes

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KCNQ1 Potassium Channel
Potassium Channels
Purification
Magnetic Resonance Spectroscopy
Voltage-Gated Potassium Channels
Sensors
Micelles
Electric potential
Deafness
Amides
Action Potentials
Cardiac Arrhythmias
Membrane Proteins
Escherichia coli
Mutation
Nuclear magnetic resonance
Proteins
Topology

ASJC Scopus subject areas

  • Biochemistry

Cite this

Peng, D., Kim, J. H., Kroncke, B. M., Law, C. L., Xia, Y., Droege, K. D., ... Sanders, C. R. (2014). Purification and structural study of the voltage-sensor domain of the human KCNQ1 potassium ion channel. Biochemistry, 53(12), 2032-2042. https://doi.org/10.1021/bi500102w

Purification and structural study of the voltage-sensor domain of the human KCNQ1 potassium ion channel. / Peng, Dungeng; Kim, Ji Hun; Kroncke, Brett M.; Law, Cheryl L.; Xia, Yan; Droege, Kristin D.; Van Horn, Wade; Vanoye, Carlos G.; Sanders, Charles R.

In: Biochemistry, Vol. 53, No. 12, 01.04.2014, p. 2032-2042.

Research output: Contribution to journalArticle

Peng, D, Kim, JH, Kroncke, BM, Law, CL, Xia, Y, Droege, KD, Van Horn, W, Vanoye, CG & Sanders, CR 2014, 'Purification and structural study of the voltage-sensor domain of the human KCNQ1 potassium ion channel', Biochemistry, vol. 53, no. 12, pp. 2032-2042. https://doi.org/10.1021/bi500102w
Peng, Dungeng ; Kim, Ji Hun ; Kroncke, Brett M. ; Law, Cheryl L. ; Xia, Yan ; Droege, Kristin D. ; Van Horn, Wade ; Vanoye, Carlos G. ; Sanders, Charles R. / Purification and structural study of the voltage-sensor domain of the human KCNQ1 potassium ion channel. In: Biochemistry. 2014 ; Vol. 53, No. 12. pp. 2032-2042.
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