TY - JOUR
T1 - PSI:Biology-materials repository
T2 - A biologist's resource for protein expression plasmids
AU - Cormier, Catherine Y.
AU - Park, Jin
AU - Fiacco, Michael
AU - Steel, Jason
AU - Hunter, Preston
AU - Kramer, Jason
AU - Singla, Rajeev
AU - LaBaer, Joshua
N1 - Funding Information:
Acknowledgments The PSI:Biology-MR team would like to thank all of the PIs and researchers in the PSI network for their collaboration, help and effort in supporting the MR. Thanks to the National Institute of General Medical Sciences (NIGMS) for funding, suggestions and support, in particular, thanks to Jean Chin and Ward Smith. Thanks also to our Advisory Board, Peter Cherbas, Cheryl Arrowsmith, Gerhard Wagner, Stephen Burley, and Stephen Bryant.
PY - 2011/7
Y1 - 2011/7
N2 - The Protein Structure Initiative:Biology-Materials Repository (PSI:Biology-MR; MR; http://psimr.asu. edu) sequence-verifies, annotates, stores, and distributes the protein expression plasmids and vectors created by the Protein Structure Initiative (PSI). The MR has developed an informatics and sample processing pipeline that manages this process for thousands of samples per month from nearly a dozen PSI centers. DNASU (http://dnasu.asu.edu), a freely searchable database, stores the plasmid annotations, which include the full-length sequence, vector information, and associated publications for over 130,000 plasmids created by our laboratory, by the PSI and other consortia, and by individual laboratories for distribution to researchers worldwide. Each plasmid links to external resources, including the PSI Structural Biology Knowledgebase (http://sbkb.org), which facilitates cross-referencing of a particular plasmid to additional protein annotations and experimental data. To expedite and simplify plasmid requests, the MR uses an expedited material transfer agreement (EP-MTA) network, where researchers from network institutions can order and receive PSI plasmids without institutional delays. As of March 2011, over 39,000 protein expression plasmids and 78 empty vectors from the PSI are available upon request from DNASU. Overall, the MR's repository of expression-ready plasmids, its automated pipeline, and the rapid process for receiving and distributing these plasmids more effectively allows the research community to dissect the biological function of proteins whose structures have been studied by the PSI.
AB - The Protein Structure Initiative:Biology-Materials Repository (PSI:Biology-MR; MR; http://psimr.asu. edu) sequence-verifies, annotates, stores, and distributes the protein expression plasmids and vectors created by the Protein Structure Initiative (PSI). The MR has developed an informatics and sample processing pipeline that manages this process for thousands of samples per month from nearly a dozen PSI centers. DNASU (http://dnasu.asu.edu), a freely searchable database, stores the plasmid annotations, which include the full-length sequence, vector information, and associated publications for over 130,000 plasmids created by our laboratory, by the PSI and other consortia, and by individual laboratories for distribution to researchers worldwide. Each plasmid links to external resources, including the PSI Structural Biology Knowledgebase (http://sbkb.org), which facilitates cross-referencing of a particular plasmid to additional protein annotations and experimental data. To expedite and simplify plasmid requests, the MR uses an expedited material transfer agreement (EP-MTA) network, where researchers from network institutions can order and receive PSI plasmids without institutional delays. As of March 2011, over 39,000 protein expression plasmids and 78 empty vectors from the PSI are available upon request from DNASU. Overall, the MR's repository of expression-ready plasmids, its automated pipeline, and the rapid process for receiving and distributing these plasmids more effectively allows the research community to dissect the biological function of proteins whose structures have been studied by the PSI.
KW - PSI:Biology
KW - Plasmid
KW - Protein expression
KW - Protein structure initiative
KW - Structural biology
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U2 - 10.1007/s10969-011-9100-8
DO - 10.1007/s10969-011-9100-8
M3 - Article
C2 - 21360289
AN - SCOPUS:80053631237
SN - 1345-711X
VL - 12
SP - 55
EP - 62
JO - Journal of Structural and Functional Genomics
JF - Journal of Structural and Functional Genomics
IS - 2
ER -