Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H+-adenosine triphosphatase

Patricia M. Kane, Carl T. Yamashiro, David F. Wolczyk, Norma Neff, Mark Goebl, Tom H. Stevens

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Abstract

The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H+-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5′ and 3′ thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.

Original languageEnglish (US)
Pages (from-to)651-657
Number of pages7
JournalScience
Volume250
Issue number4981
DOIs
StatePublished - Jan 1 1990

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Cite this

Kane, P. M., Yamashiro, C. T., Wolczyk, D. F., Neff, N., Goebl, M., & Stevens, T. H. (1990). Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H+-adenosine triphosphatase. Science, 250(4981), 651-657. https://doi.org/10.1126/science.2146742