Protein Expression Library Resources for Proteome Studies

Joshua LaBaer, Gerald Marsischky

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

There are several public projects worldwide with the goal of creating representative full-length cDNA clone collections, notably the NIH Mammalian Gene Collection (MGC) project and the Mouse Gene Encyclopedia Project sponsored by the RIKEN Institute in Japan. These projects have taken the approach of sifting through large numbers of cDNA clones, e.g. from tissue-specific libraries, to identify unique full-length cDNA clones. There are, additionally, independent efforts to create ORF clone collections for use as expression tools. These include the FLEXGene effort led by the Institute of Proteomics at Harvard Medical School (HIP), the Caenorhabditis elegans ORFeome Project, and the Ressourcenzentrum fûr Genomforschung (RZPD) ORF clone collection. These clone collections are available at this time as either cDNA clones or ORF clones. While cDNA clones are the most straightforward to create, they have important limitations as experimental tools. The cloned sequences serve not only as a tool to assist in the assignment of gene boundaries and the identification of splice isoforms, but also serve as the starting point for a new kind of genome-scale tool: an arrayed, sequence-validated, and full-length ORF clone collection that is highly representative of the gene content of an organism. These types of collections are under development for human, mouse, several of the important model organisms, and an important human pathogen, P. aeruginosa.

Original languageEnglish (US)
Title of host publicationProteome Analysis: Interpreting the Genome
PublisherElsevier B.V.
Pages287-304
Number of pages18
ISBN (Print)9780444510242
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Proteome
Complementary DNA
Clone Cells
Genes
Proteins
Open Reading Frames
Hot isostatic pressing
Pathogens
Protein Isoforms
Encyclopedias
Tissue
Caenorhabditis elegans
Human Development
Medical Schools
Proteomics
Libraries
Japan
Genome

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

LaBaer, J., & Marsischky, G. (2004). Protein Expression Library Resources for Proteome Studies. In Proteome Analysis: Interpreting the Genome (pp. 287-304). Elsevier B.V.. https://doi.org/10.1016/B978-044451024-2/50028-X

Protein Expression Library Resources for Proteome Studies. / LaBaer, Joshua; Marsischky, Gerald.

Proteome Analysis: Interpreting the Genome. Elsevier B.V., 2004. p. 287-304.

Research output: Chapter in Book/Report/Conference proceedingChapter

LaBaer, J & Marsischky, G 2004, Protein Expression Library Resources for Proteome Studies. in Proteome Analysis: Interpreting the Genome. Elsevier B.V., pp. 287-304. https://doi.org/10.1016/B978-044451024-2/50028-X
LaBaer J, Marsischky G. Protein Expression Library Resources for Proteome Studies. In Proteome Analysis: Interpreting the Genome. Elsevier B.V. 2004. p. 287-304 https://doi.org/10.1016/B978-044451024-2/50028-X
LaBaer, Joshua ; Marsischky, Gerald. / Protein Expression Library Resources for Proteome Studies. Proteome Analysis: Interpreting the Genome. Elsevier B.V., 2004. pp. 287-304
@inbook{d0c76a883d7343c7880c2d60019ed041,
title = "Protein Expression Library Resources for Proteome Studies",
abstract = "There are several public projects worldwide with the goal of creating representative full-length cDNA clone collections, notably the NIH Mammalian Gene Collection (MGC) project and the Mouse Gene Encyclopedia Project sponsored by the RIKEN Institute in Japan. These projects have taken the approach of sifting through large numbers of cDNA clones, e.g. from tissue-specific libraries, to identify unique full-length cDNA clones. There are, additionally, independent efforts to create ORF clone collections for use as expression tools. These include the FLEXGene effort led by the Institute of Proteomics at Harvard Medical School (HIP), the Caenorhabditis elegans ORFeome Project, and the Ressourcenzentrum f{\^u}r Genomforschung (RZPD) ORF clone collection. These clone collections are available at this time as either cDNA clones or ORF clones. While cDNA clones are the most straightforward to create, they have important limitations as experimental tools. The cloned sequences serve not only as a tool to assist in the assignment of gene boundaries and the identification of splice isoforms, but also serve as the starting point for a new kind of genome-scale tool: an arrayed, sequence-validated, and full-length ORF clone collection that is highly representative of the gene content of an organism. These types of collections are under development for human, mouse, several of the important model organisms, and an important human pathogen, P. aeruginosa.",
author = "Joshua LaBaer and Gerald Marsischky",
year = "2004",
month = "3",
doi = "10.1016/B978-044451024-2/50028-X",
language = "English (US)",
isbn = "9780444510242",
pages = "287--304",
booktitle = "Proteome Analysis: Interpreting the Genome",
publisher = "Elsevier B.V.",

}

TY - CHAP

T1 - Protein Expression Library Resources for Proteome Studies

AU - LaBaer, Joshua

AU - Marsischky, Gerald

PY - 2004/3

Y1 - 2004/3

N2 - There are several public projects worldwide with the goal of creating representative full-length cDNA clone collections, notably the NIH Mammalian Gene Collection (MGC) project and the Mouse Gene Encyclopedia Project sponsored by the RIKEN Institute in Japan. These projects have taken the approach of sifting through large numbers of cDNA clones, e.g. from tissue-specific libraries, to identify unique full-length cDNA clones. There are, additionally, independent efforts to create ORF clone collections for use as expression tools. These include the FLEXGene effort led by the Institute of Proteomics at Harvard Medical School (HIP), the Caenorhabditis elegans ORFeome Project, and the Ressourcenzentrum fûr Genomforschung (RZPD) ORF clone collection. These clone collections are available at this time as either cDNA clones or ORF clones. While cDNA clones are the most straightforward to create, they have important limitations as experimental tools. The cloned sequences serve not only as a tool to assist in the assignment of gene boundaries and the identification of splice isoforms, but also serve as the starting point for a new kind of genome-scale tool: an arrayed, sequence-validated, and full-length ORF clone collection that is highly representative of the gene content of an organism. These types of collections are under development for human, mouse, several of the important model organisms, and an important human pathogen, P. aeruginosa.

AB - There are several public projects worldwide with the goal of creating representative full-length cDNA clone collections, notably the NIH Mammalian Gene Collection (MGC) project and the Mouse Gene Encyclopedia Project sponsored by the RIKEN Institute in Japan. These projects have taken the approach of sifting through large numbers of cDNA clones, e.g. from tissue-specific libraries, to identify unique full-length cDNA clones. There are, additionally, independent efforts to create ORF clone collections for use as expression tools. These include the FLEXGene effort led by the Institute of Proteomics at Harvard Medical School (HIP), the Caenorhabditis elegans ORFeome Project, and the Ressourcenzentrum fûr Genomforschung (RZPD) ORF clone collection. These clone collections are available at this time as either cDNA clones or ORF clones. While cDNA clones are the most straightforward to create, they have important limitations as experimental tools. The cloned sequences serve not only as a tool to assist in the assignment of gene boundaries and the identification of splice isoforms, but also serve as the starting point for a new kind of genome-scale tool: an arrayed, sequence-validated, and full-length ORF clone collection that is highly representative of the gene content of an organism. These types of collections are under development for human, mouse, several of the important model organisms, and an important human pathogen, P. aeruginosa.

UR - http://www.scopus.com/inward/record.url?scp=84904079108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904079108&partnerID=8YFLogxK

U2 - 10.1016/B978-044451024-2/50028-X

DO - 10.1016/B978-044451024-2/50028-X

M3 - Chapter

SN - 9780444510242

SP - 287

EP - 304

BT - Proteome Analysis: Interpreting the Genome

PB - Elsevier B.V.

ER -