Protection against avian metapneumovirus subtype C in turkeys immunized via the respiratory tract with inactivated virus

Ra Mi Cha, Mahesh Khatri, Jagdev M. Sharma

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Avian metapneumovirus subtype C (aMPV/C) causes a severe upper respiratory tract (URT) infection in turkeys. Turkeys were inoculated oculonasally with inactivated aMPV/C adjuvanted with synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid (Poly IC). Immunized turkeys had elevated numbers of mucosal IgA+ cells in the URT and increased levels of virus-specific IgG and IgA in the lachrymal fluid and IgG in the serum. After 7 or 21 days post immunization, turkeys were challenged oculonasally with pathogenic aMPV/C. Immunized groups were protected against respiratory lesions induced by the challenge virus. Further, the viral copy number of the challenge virus in the URT were significantly lower in the immunized turkeys than in the unimmunized turkeys (P<0.05). These results showed that inactivated aMPV/C administered by the respiratory route induced protective immunity against pathogenic virus challenge.

Original languageEnglish (US)
Pages (from-to)459-465
Number of pages7
JournalVaccine
Volume29
Issue number3
DOIs
StatePublished - Jan 10 2011
Externally publishedYes

Fingerprint

Metapneumovirus
Avian metapneumovirus
respiratory system
Respiratory System
Viruses
viruses
Immunoglobulin A
Immunoglobulin G
Double-Stranded RNA
double-stranded RNA
lesions (animal)
Respiratory Tract Infections
respiratory tract diseases
Immunity
Immunization
immunization
immunity
acids
Serum

Keywords

  • Avian metapneumovirus
  • Inactivated vaccine
  • Mucosal immunity
  • Poly IC
  • Turkeys
  • Upper respiratory tract

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Protection against avian metapneumovirus subtype C in turkeys immunized via the respiratory tract with inactivated virus. / Cha, Ra Mi; Khatri, Mahesh; Sharma, Jagdev M.

In: Vaccine, Vol. 29, No. 3, 10.01.2011, p. 459-465.

Research output: Contribution to journalArticle

Cha, Ra Mi ; Khatri, Mahesh ; Sharma, Jagdev M. / Protection against avian metapneumovirus subtype C in turkeys immunized via the respiratory tract with inactivated virus. In: Vaccine. 2011 ; Vol. 29, No. 3. pp. 459-465.
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