Properties of Purified Ribonuclease P from Escherichia coli

Ryszard Kole, Sidney Altman

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The purified protein moiety of ribonuclease P. (EC 3.1.26.5) from Escherichia coli, a single polypeptide of molecular weight ~ 17 500, has no catalytic activity by itself on several RNA substrates. However, when it is mixed in vitro with an RNA species called M1 RNA, RNase P activity is reconstituted. The rate at which the purified RNase P cleaves any particular tRNA precursor molecule depends on the identity of that tRNA precursor.

Original languageEnglish (US)
Pages (from-to)1902-1906
Number of pages5
JournalBiochemistry
Volume20
Issue number7
DOIs
StatePublished - Mar 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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