TY - JOUR
T1 - Promoting Synechocystis sp. PCC 6803 Harvesting by Cationic Surfactants
T2 - Alkyl-Chain Length and Dose Control for the Release of Extracellular Polymeric Substances and Biomass Aggregation
AU - Zhou, Yun
AU - Lai, Yenjung Sean
AU - Eustance, Everett
AU - Rittmann, Bruce
N1 - Funding Information:
This work was supported by LightWorks, Arizona State University, and Shanghai Tongji Gao Tingyao Environmental Science & Technology Development Foundation. We thank Dr. Willem Vermaas and his laboratory in the School of Life Sciences at Arizona State University for providing Synechocystis sp. PCC6803 wild type and Dr. Dong Fu at the Center of Infectious Diseases and Vaccinology, Biodesign Institute at Arizona State University, for her expertise in flow cytometry for sample quantification.
Publisher Copyright:
© 2018 American Chemical Society.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/1/22
Y1 - 2019/1/22
N2 - Development of an efficient biomass-harvesting technology for microalgae would achieve cost and energy savings in large-scale microalgae biomass cultivation. Cationic surfactants could improve biomass harvesting, but determining the optimal type and dose of surfactant requires mechanistic understanding. In this study, we evaluated how the alkyl-chain length and dose of three cationic surfactants - hexadecyltrimethylammonium bromide (CTAB), myristyltrimethylammonium bromide (MTAB), and dodecyltrimethylammonium bromide (DTAB) - affected biomass harvesting of Synechocystis. Flow cytometry (FC) with the nucleic-acid (NA) stain SYTOX Green (SG) was used to differentiate the release of extracellular polymeric substances (EPS) from cell lysis. All selected cationic surfactants dramatically improved the biomass-harvesting efficiency, and harvesting kinetics were represented well with a first-order kinetic model. The efficiency of biomass harvesting correlated positively with the alkyl-chain length; i.e., CTAB > MTAB > DTAB. A longer alkyl chain increased EPS release, which made it easier to achieve a less-negative zeta potential but without cell lysis. For CTAB, the largest cationic surfactant tested, a dose of 4.5 mM and treatment for 60 min achieved the maximum harvesting efficiency of ∼91%. This work lays the foundation for optimizing surfactant species and dose for biomass harvesting.
AB - Development of an efficient biomass-harvesting technology for microalgae would achieve cost and energy savings in large-scale microalgae biomass cultivation. Cationic surfactants could improve biomass harvesting, but determining the optimal type and dose of surfactant requires mechanistic understanding. In this study, we evaluated how the alkyl-chain length and dose of three cationic surfactants - hexadecyltrimethylammonium bromide (CTAB), myristyltrimethylammonium bromide (MTAB), and dodecyltrimethylammonium bromide (DTAB) - affected biomass harvesting of Synechocystis. Flow cytometry (FC) with the nucleic-acid (NA) stain SYTOX Green (SG) was used to differentiate the release of extracellular polymeric substances (EPS) from cell lysis. All selected cationic surfactants dramatically improved the biomass-harvesting efficiency, and harvesting kinetics were represented well with a first-order kinetic model. The efficiency of biomass harvesting correlated positively with the alkyl-chain length; i.e., CTAB > MTAB > DTAB. A longer alkyl chain increased EPS release, which made it easier to achieve a less-negative zeta potential but without cell lysis. For CTAB, the largest cationic surfactant tested, a dose of 4.5 mM and treatment for 60 min achieved the maximum harvesting efficiency of ∼91%. This work lays the foundation for optimizing surfactant species and dose for biomass harvesting.
KW - Biomass harvesting
KW - Cationic surfactants
KW - Cyanobacteria
KW - Flow cytometry
KW - Synechocystis
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U2 - 10.1021/acssuschemeng.8b04776
DO - 10.1021/acssuschemeng.8b04776
M3 - Article
AN - SCOPUS:85059814655
SN - 2168-0485
VL - 7
SP - 2127
EP - 2133
JO - ACS Sustainable Chemistry and Engineering
JF - ACS Sustainable Chemistry and Engineering
IS - 2
ER -