Abstract

Development of an efficient biomass-harvesting technology for microalgae would achieve cost and energy savings in large-scale microalgae biomass cultivation. Cationic surfactants could improve biomass harvesting, but determining the optimal type and dose of surfactant requires mechanistic understanding. In this study, we evaluated how the alkyl-chain length and dose of three cationic surfactants - hexadecyltrimethylammonium bromide (CTAB), myristyltrimethylammonium bromide (MTAB), and dodecyltrimethylammonium bromide (DTAB) - affected biomass harvesting of Synechocystis. Flow cytometry (FC) with the nucleic-acid (NA) stain SYTOX Green (SG) was used to differentiate the release of extracellular polymeric substances (EPS) from cell lysis. All selected cationic surfactants dramatically improved the biomass-harvesting efficiency, and harvesting kinetics were represented well with a first-order kinetic model. The efficiency of biomass harvesting correlated positively with the alkyl-chain length; i.e., CTAB > MTAB > DTAB. A longer alkyl chain increased EPS release, which made it easier to achieve a less-negative zeta potential but without cell lysis. For CTAB, the largest cationic surfactant tested, a dose of 4.5 mM and treatment for 60 min achieved the maximum harvesting efficiency of ∼91%. This work lays the foundation for optimizing surfactant species and dose for biomass harvesting.

Original languageEnglish (US)
Pages (from-to)2127-2133
Number of pages7
JournalACS Sustainable Chemistry and Engineering
Volume7
Issue number2
DOIs
StatePublished - Jan 22 2019

Fingerprint

Cationic surfactants
Chain length
surfactant
Biomass
Agglomeration
bromide
biomass
lysis
Surface-Active Agents
Surface active agents
kinetics
Kinetics
Flow cytometry
dose
Nucleic acids
flow cytometry
nucleic acid
Zeta potential
Bromides
Nucleic Acids

Keywords

  • Biomass harvesting
  • Cationic surfactants
  • Cyanobacteria
  • Flow cytometry
  • Synechocystis

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment

Cite this

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title = "Promoting Synechocystis sp. PCC 6803 Harvesting by Cationic Surfactants: Alkyl-Chain Length and Dose Control for the Release of Extracellular Polymeric Substances and Biomass Aggregation",
abstract = "Development of an efficient biomass-harvesting technology for microalgae would achieve cost and energy savings in large-scale microalgae biomass cultivation. Cationic surfactants could improve biomass harvesting, but determining the optimal type and dose of surfactant requires mechanistic understanding. In this study, we evaluated how the alkyl-chain length and dose of three cationic surfactants - hexadecyltrimethylammonium bromide (CTAB), myristyltrimethylammonium bromide (MTAB), and dodecyltrimethylammonium bromide (DTAB) - affected biomass harvesting of Synechocystis. Flow cytometry (FC) with the nucleic-acid (NA) stain SYTOX Green (SG) was used to differentiate the release of extracellular polymeric substances (EPS) from cell lysis. All selected cationic surfactants dramatically improved the biomass-harvesting efficiency, and harvesting kinetics were represented well with a first-order kinetic model. The efficiency of biomass harvesting correlated positively with the alkyl-chain length; i.e., CTAB > MTAB > DTAB. A longer alkyl chain increased EPS release, which made it easier to achieve a less-negative zeta potential but without cell lysis. For CTAB, the largest cationic surfactant tested, a dose of 4.5 mM and treatment for 60 min achieved the maximum harvesting efficiency of ∼91{\%}. This work lays the foundation for optimizing surfactant species and dose for biomass harvesting.",
keywords = "Biomass harvesting, Cationic surfactants, Cyanobacteria, Flow cytometry, Synechocystis",
author = "Yun Zhou and Lai, {Yenjung Sean} and Everett Eustance and Bruce Rittmann",
year = "2019",
month = "1",
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TY - JOUR

T1 - Promoting Synechocystis sp. PCC 6803 Harvesting by Cationic Surfactants

T2 - Alkyl-Chain Length and Dose Control for the Release of Extracellular Polymeric Substances and Biomass Aggregation

AU - Zhou, Yun

AU - Lai, Yenjung Sean

AU - Eustance, Everett

AU - Rittmann, Bruce

PY - 2019/1/22

Y1 - 2019/1/22

N2 - Development of an efficient biomass-harvesting technology for microalgae would achieve cost and energy savings in large-scale microalgae biomass cultivation. Cationic surfactants could improve biomass harvesting, but determining the optimal type and dose of surfactant requires mechanistic understanding. In this study, we evaluated how the alkyl-chain length and dose of three cationic surfactants - hexadecyltrimethylammonium bromide (CTAB), myristyltrimethylammonium bromide (MTAB), and dodecyltrimethylammonium bromide (DTAB) - affected biomass harvesting of Synechocystis. Flow cytometry (FC) with the nucleic-acid (NA) stain SYTOX Green (SG) was used to differentiate the release of extracellular polymeric substances (EPS) from cell lysis. All selected cationic surfactants dramatically improved the biomass-harvesting efficiency, and harvesting kinetics were represented well with a first-order kinetic model. The efficiency of biomass harvesting correlated positively with the alkyl-chain length; i.e., CTAB > MTAB > DTAB. A longer alkyl chain increased EPS release, which made it easier to achieve a less-negative zeta potential but without cell lysis. For CTAB, the largest cationic surfactant tested, a dose of 4.5 mM and treatment for 60 min achieved the maximum harvesting efficiency of ∼91%. This work lays the foundation for optimizing surfactant species and dose for biomass harvesting.

AB - Development of an efficient biomass-harvesting technology for microalgae would achieve cost and energy savings in large-scale microalgae biomass cultivation. Cationic surfactants could improve biomass harvesting, but determining the optimal type and dose of surfactant requires mechanistic understanding. In this study, we evaluated how the alkyl-chain length and dose of three cationic surfactants - hexadecyltrimethylammonium bromide (CTAB), myristyltrimethylammonium bromide (MTAB), and dodecyltrimethylammonium bromide (DTAB) - affected biomass harvesting of Synechocystis. Flow cytometry (FC) with the nucleic-acid (NA) stain SYTOX Green (SG) was used to differentiate the release of extracellular polymeric substances (EPS) from cell lysis. All selected cationic surfactants dramatically improved the biomass-harvesting efficiency, and harvesting kinetics were represented well with a first-order kinetic model. The efficiency of biomass harvesting correlated positively with the alkyl-chain length; i.e., CTAB > MTAB > DTAB. A longer alkyl chain increased EPS release, which made it easier to achieve a less-negative zeta potential but without cell lysis. For CTAB, the largest cationic surfactant tested, a dose of 4.5 mM and treatment for 60 min achieved the maximum harvesting efficiency of ∼91%. This work lays the foundation for optimizing surfactant species and dose for biomass harvesting.

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KW - Flow cytometry

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