Production of a fusion protein consisting of the enterotoxigenic Escherichia coli heat-labile toxin B subunit and a tuberculosis antigen in Arabidopsis thaliana

M. M. Rigano, M. L. Alvarez, J. Pinkhasov, Y. Jin, F. Sala, C. J. Arntzen, A. M. Walmsley

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Transgenic plants are potentially safe and inexpensive vehicles to produce and mucosally deliver protective antigens. However, the application of this technology is limited by the poor response of the immune system to non-particulate, subunit vaccines. Co-delivery of therapeutic proteins with carrier proteins could increase the effectiveness of the antigen. This paper reports the ability of transgenic Arabidopsis thaliana plants to produce a fusion protein consisting of the B subunit of the Escherichia coli heat-labile enterotoxin and a 6 kDa tuberculosis antigen, the early secretory antigenic target ESAT-6. Both components of the fusion protein were detected using GM1-ganglioside-dependent enzyme-linked immunosorbant assay. This suggested the fusion protein retained both its native antigenicity and the ability to form pentamers.

Original languageEnglish (US)
Pages (from-to)502-508
Number of pages7
JournalPlant Cell Reports
Volume22
Issue number7
DOIs
StatePublished - Feb 2004

Fingerprint

enterotoxigenic Escherichia coli
tuberculosis
toxins
Arabidopsis thaliana
antigens
heat
biopharmaceuticals
gangliosides
application technology
proteins
subunit vaccines
transport proteins
enterotoxins
immune system
transgenic plants
genetically modified organisms
Escherichia coli
assays
enzymes

Keywords

  • Carrier protein
  • LTB fusion protein
  • Mucosal delivery
  • Transgenic plants
  • Tuberculosis vaccine

ASJC Scopus subject areas

  • Plant Science

Cite this

Production of a fusion protein consisting of the enterotoxigenic Escherichia coli heat-labile toxin B subunit and a tuberculosis antigen in Arabidopsis thaliana. / Rigano, M. M.; Alvarez, M. L.; Pinkhasov, J.; Jin, Y.; Sala, F.; Arntzen, C. J.; Walmsley, A. M.

In: Plant Cell Reports, Vol. 22, No. 7, 02.2004, p. 502-508.

Research output: Contribution to journalArticle

Rigano, M. M. ; Alvarez, M. L. ; Pinkhasov, J. ; Jin, Y. ; Sala, F. ; Arntzen, C. J. ; Walmsley, A. M. / Production of a fusion protein consisting of the enterotoxigenic Escherichia coli heat-labile toxin B subunit and a tuberculosis antigen in Arabidopsis thaliana. In: Plant Cell Reports. 2004 ; Vol. 22, No. 7. pp. 502-508.
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