Pmp27 promotes peroxisomal proliferation

Pamela Marshall, Y. I. Krimkevich, R. H. Lark, J. M. Dyer, M. Veenhuis, J. M. Goodman

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

Peroxisomes perform many essential functions in eukaryotic cells. The weight of evidence indicates that these organelles divide by budding from preexisting peroxisomes. This process is not understood at the molecular level. Peroxisomal proliferation can be induced in Saccharomyces cerevisiae by oleate. This growth substrate is metabolized by peroxisomal enzymes. We have identified a protein, Prop27, that promotes peroxisomal proliferation. This protein, previously termed Pmp24, was purified from peroxisomal membranes, and the corresponding gene, Pmp27, was isolated and sequenced. Pmp27 shares sequence similarity with the Pmp30 family in Candida boidinii. Pmp27 is a hydrophobic peroxisomal membrane protein but it can be extracted by high pH, suggesting that it does not fully span the bilayer. Its expression is regulated by oleate. The function of Pmp27 was probed by observing the phenotype of strains in which the protein was eliminated by gene disruption or overproduced by expression from a multicopy plasmid. The strain containing the disruption (3B) was able to grow on all carbon sources tested, including oleate, although growth on oleate, glycerol, and acetate was slower than wild type. Strain 3B contained peroxisomes with all of the enzymes of β-oxidation. However, in addition to the presence of a few modestly sized peroxisomes seen in a typical thin section of a cell growing on oleate-containing medium, cells of strain 3B also contained one or two very large peroxisomes. In contrast, cells in a strain in which Pmp27 was overexpressed contained an increased number of normal-sized peroxisomes. We suggest that Pmp27 promotes peroxisomal proliferation by participating in peroxisomal elongation or fission.

Original languageEnglish (US)
Pages (from-to)345-355
Number of pages11
JournalJournal of Cell Biology
Volume129
Issue number2
StatePublished - 1995
Externally publishedYes

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Peroxisomes
Oleic Acid
Proteins
Eukaryotic Cells
Enzymes
Growth
Candida
Organelles
Glycerol
Genes
Saccharomyces cerevisiae
Membrane Proteins
Acetates
Plasmids
Carbon
Phenotype
Weights and Measures
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Marshall, P., Krimkevich, Y. I., Lark, R. H., Dyer, J. M., Veenhuis, M., & Goodman, J. M. (1995). Pmp27 promotes peroxisomal proliferation. Journal of Cell Biology, 129(2), 345-355.

Pmp27 promotes peroxisomal proliferation. / Marshall, Pamela; Krimkevich, Y. I.; Lark, R. H.; Dyer, J. M.; Veenhuis, M.; Goodman, J. M.

In: Journal of Cell Biology, Vol. 129, No. 2, 1995, p. 345-355.

Research output: Contribution to journalArticle

Marshall, P, Krimkevich, YI, Lark, RH, Dyer, JM, Veenhuis, M & Goodman, JM 1995, 'Pmp27 promotes peroxisomal proliferation', Journal of Cell Biology, vol. 129, no. 2, pp. 345-355.
Marshall P, Krimkevich YI, Lark RH, Dyer JM, Veenhuis M, Goodman JM. Pmp27 promotes peroxisomal proliferation. Journal of Cell Biology. 1995;129(2):345-355.
Marshall, Pamela ; Krimkevich, Y. I. ; Lark, R. H. ; Dyer, J. M. ; Veenhuis, M. ; Goodman, J. M. / Pmp27 promotes peroxisomal proliferation. In: Journal of Cell Biology. 1995 ; Vol. 129, No. 2. pp. 345-355.
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