@article{12b9df631ecb412683735a5e170543b5,
title = "Pink-beam serial crystallography",
abstract = "Serial X-ray crystallography allows macromolecular structure determination at both X-ray free electron lasers (XFELs) and, more recently, synchrotron sources. The time resolution for serial synchrotron crystallography experiments has been limited to millisecond timescales with monochromatic beams. The polychromatic, {"}pink{"}, beam provides a more than two orders of magnitude increased photon flux and hence allows accessing much shorter timescales in diffraction experiments at synchrotron sources. Here we report the structure determination of two different protein samples by merging pink-beam diffraction patterns from many crystals, each collected with a single 100 ps X-ray pulse exposure per crystal using a setup optimized for very low scattering background. In contrast to experiments with monochromatic radiation, data from only 50 crystals were required to obtain complete datasets. The high quality of the diffraction data highlights the potential of this method for studying irreversible reactions at sub-microsecond timescales using high-brightness X-ray facilities.",
author = "A. Meents and Wiedorn, {M. O.} and V. Srajer and R. Henning and I. Sarrou and J. Bergtholdt and M. Barthelmess and Reinke, {P. Y.A.} and D. Dierksmeyer and A. Tolstikova and S. Schaible and M. Messerschmidt and Ogata, {C. M.} and Kissick, {D. J.} and Taft, {M. H.} and Manstein, {D. J.} and J. Lieske and D. Oberthuer and Fischetti, {R. F.} and Chapman, {H. N.}",
note = "Funding Information: This work was funded as part of the European Cluster of Advanced Laser Light Sources (EUCALL) project which has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under Grant Agreement No. 654220. This work was further supported by the European Research Council under the European Union Seventh Framework Program (FP/2007-2013)/ERC Grant Agreement No. 609920, “X-probe” funded by the European Union{\textquoteright}s 2020 Research and Innovation Program under the Marie Sk{\l}odowska-Curie Grant Agreement 637295, BMBF project 05K14CHA “Sync-FELMed”, and the Virtual Institutes VI-403 and VI-419 of the Helmholtz Association. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. Use of Bio-CARS was also supported by the National Institute of General Medical Sciences of the National Institutes of Health under grant number R24GM111072. Time-resolved setup at Sector 14 was funded in part through a collaboration with Philip Anfinrud (NIH/ NIDDK). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41467-017-01417-3",
language = "English (US)",
volume = "8",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}