Phylogenetic placement, genome size, and gc content of the liquid-hydrocarbon-producing green microalga botryococcus braunii strain berkeley (showa) (chlorophyta)

Taylor L. Weiss, J. Spencer Johnston, Kazuhiro Fujisawa, Koremitsu Sumimoto, Shigeru Okada, Joe Chappell, Timothy P. Devarenne

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

We report the genome size and the GC content, and perform a phylogenetic analysis on Botryococcus braunii Kütz., a green, colony-forming, hydrocarbon-rich alga that is an attractive source for biopetroleum. While the chemistry of the hydrocarbons produced by the B race of B. braunii has been studied for many years, there is a deficiency of information concerning the molecular biology of this alga. In addition, there has been some discrepancy as to the phylogenetic placement of the Berkeley (or Showa) strain of the B race. To clarify its classification, we isolated the Berkeley strain nuclear SSU (18S) rRNA gene and β-actin cDNA and used these sequences for phylogenetic analysis to determine that the Berkeley strain belongs to the Trebouxiophyceae class. This finding is in agreement with other B races of B. braunii, indicating the Berkeley strain is a true B race of B. braunii. To better understand molecular aspects of B. braunii, we obtained the Berkeley strain genome size as a first step in genome sequencing. Using flow cytometry, we determined the B. braunii Berkeley genome size to be 166.2 ± 2.2 Mb. We also estimated the GC content of the Berkeley strain as 54.4 ± 1.2% for expressed gene sequences.

Original languageEnglish (US)
Pages (from-to)534-540
Number of pages7
JournalJournal of Phycology
Volume46
Issue number3
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

Keywords

  • 18S rRNA sequences
  • Botryococcus braunii
  • Chlorophyta
  • GC content
  • Genome size
  • Hydrocarbons
  • Phylogenetic analysis
  • Trebouxiophyceae

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

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