Phylogenetic placement, genome size, and gc content of the liquid-hydrocarbon-producing green microalga botryococcus braunii strain berkeley (showa) (chlorophyta)

Taylor Weiss, J. Spencer Johnston, Kazuhiro Fujisawa, Koremitsu Sumimoto, Shigeru Okada, Joe Chappell, Timothy P. Devarenne

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We report the genome size and the GC content, and perform a phylogenetic analysis on Botryococcus braunii Kütz., a green, colony-forming, hydrocarbon-rich alga that is an attractive source for biopetroleum. While the chemistry of the hydrocarbons produced by the B race of B. braunii has been studied for many years, there is a deficiency of information concerning the molecular biology of this alga. In addition, there has been some discrepancy as to the phylogenetic placement of the Berkeley (or Showa) strain of the B race. To clarify its classification, we isolated the Berkeley strain nuclear SSU (18S) rRNA gene and β-actin cDNA and used these sequences for phylogenetic analysis to determine that the Berkeley strain belongs to the Trebouxiophyceae class. This finding is in agreement with other B races of B. braunii, indicating the Berkeley strain is a true B race of B. braunii. To better understand molecular aspects of B. braunii, we obtained the Berkeley strain genome size as a first step in genome sequencing. Using flow cytometry, we determined the B. braunii Berkeley genome size to be 166.2 ± 2.2 Mb. We also estimated the GC content of the Berkeley strain as 54.4 ± 1.2% for expressed gene sequences.

Original languageEnglish (US)
Pages (from-to)534-540
Number of pages7
JournalJournal of Phycology
Volume46
Issue number3
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

Fingerprint

microalga
Chlorophyta
hydrocarbons
genome
hydrocarbon
phylogenetics
algae
liquid
liquids
phylogeny
alga
gene
flow cytometry
molecular biology
actin
Botryococcus braunii
chemistry
ribosomal RNA
taxonomy
nucleotide sequences

Keywords

  • 18S rRNA sequences
  • Botryococcus braunii
  • Chlorophyta
  • GC content
  • Genome size
  • Hydrocarbons
  • Phylogenetic analysis
  • Trebouxiophyceae

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Phylogenetic placement, genome size, and gc content of the liquid-hydrocarbon-producing green microalga botryococcus braunii strain berkeley (showa) (chlorophyta). / Weiss, Taylor; Spencer Johnston, J.; Fujisawa, Kazuhiro; Sumimoto, Koremitsu; Okada, Shigeru; Chappell, Joe; Devarenne, Timothy P.

In: Journal of Phycology, Vol. 46, No. 3, 01.06.2010, p. 534-540.

Research output: Contribution to journalArticle

Weiss, Taylor ; Spencer Johnston, J. ; Fujisawa, Kazuhiro ; Sumimoto, Koremitsu ; Okada, Shigeru ; Chappell, Joe ; Devarenne, Timothy P. / Phylogenetic placement, genome size, and gc content of the liquid-hydrocarbon-producing green microalga botryococcus braunii strain berkeley (showa) (chlorophyta). In: Journal of Phycology. 2010 ; Vol. 46, No. 3. pp. 534-540.
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