Phosphorylation of plant eukaryotic initiation factor-2 by the plant- encoded double-stranded RNA-dependent protein kinase, pPKR, and inhibition of protein synthesis in vitro

Jeffrey Langland, L. A. Langland, K. S. Browning, D. A. Roth

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Regulation of protein synthesis by eukaryotic initiation factor-2α (eIF- 2α) phosphorylation is a highly conserved phenomenon in eukaryotes that occurs in response to various stress conditions. Protein kinases capable of phosphorylating eIF-2α have been characterized from mammals and yeast. However, the phenomenon of eIF2-α-mediated regulation of protein synthesis and the presence of an eIF-2α kinase has not been demonstrated in higher plants. We show that plant eIF-2α (peIF-2α) and mammalian eIF-2α (meIF- 2α) are phosphorylated similarly by both the double-stranded RNA-binding kinase, pPKR, present in plant ribosome salt wash fractions and the meIF-2α kinase, PKR. By several criteria, phosphorylation of peIF-2α is directly correlated with pPKR protein and autophosphorylation levels. Significantly, pPKR is capable of specifically phosphorylating Ser51 in a synthetic eIF- 2α peptide, a key characteristic of the eIF-2α kinase family. Taken together, these data support the concept that pKR is a member of the eIF-2α kinase family. In addition, the inhibition of brome mosaic virus RNA in vitro translation in wheat germ lysates by the addition of double-stranded RNA, phosphorylated peIF-2α, meIF-2α, or activated human PKR suggests that plant protein synthesis may be regulated via phosphorylation of eIF-2α.

Original languageEnglish (US)
Pages (from-to)4539-4544
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number8
DOIs
StatePublished - Mar 7 1996
Externally publishedYes

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Eukaryotic Initiation Factor-2
eIF-2 Kinase
Phosphorylation
Double-Stranded RNA
Phosphotransferases
Plant Proteins
Proteins
Mammals
Viruses
Yeast
Protein Kinases
Bromovirus
Salts
RNA
Eukaryota
Ribosomes
Triticum
Yeasts
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphorylation of plant eukaryotic initiation factor-2 by the plant- encoded double-stranded RNA-dependent protein kinase, pPKR, and inhibition of protein synthesis in vitro. / Langland, Jeffrey; Langland, L. A.; Browning, K. S.; Roth, D. A.

In: Journal of Biological Chemistry, Vol. 271, No. 8, 07.03.1996, p. 4539-4544.

Research output: Contribution to journalArticle

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