Passion fruit chlorotic mottle virus: Molecular characterization of a new divergent geminivirus in Brazil

Rafaela S. Fontenele, Rayane A. Abreu, Natalia S. Lamas, Dione M.T. Alves-Freitas, Andreza H. Vidal, Raul R. Poppiel, Fernando L. Melo, Cristiano Lacorte, Darren P. Martin, Magnolia A. Campos, Arvind Varsani, Simone G. Ribeiro

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Brazil is one of the major passion fruit producers worldwide. Viral diseases are among the most important constraints for passion fruit production. Here we identify and characterize a new passion fruit infecting-virus belonging to the family Geminiviridae: passion fruit chlorotic mottle virus (PCMoV). PCMoV is a divergent geminivirus unlike previously characterized passion fruit-infecting geminiviruses that belonged to the genus Begomovirus. Among the presently known geminiviruses, it is most closely related to, and shares ~62% genome-wide identity with citrus chlorotic dwarf associated virus (CCDaV) and camelia chlorotic dwarf associated virus (CaCDaV). The 3743 nt PCMoV genome encodes a capsid protein (CP) and replication-associated protein (Rep) that respectively share 56 and 60% amino acid identity with those encoded by CaCDaV. The CPs of PCMoV, CCDaV, and CaCDaV cluster with those of begomovirus whereas their Reps with those of becurtoviruses. Hence, these viruses likely represent a lineage of recombinant begomo-like and becurto-like ancestral viruses. Furthermore, PCMoV, CCDaV, and CaCDaV genomes are ~12–30% larger than monopartite geminiviruses and this is primarily due to the encoded movement protein (MP; 891–921 nt) and this MP is most closely related to that encoded by the DNA-B component of bipartite begomoviruses. Hence, PCMoV, CCDaV, and CaCDaV lineage of viruses may represent molecules in an intermediary step in the evolution of bipartite begomoviruses (~5.3 kb) from monopartite geminiviruses (~2.7–3 kb). An infectious clone of PCMoV systemically infected Nicotiana benthamina, Arabidopsis thaliana, and Passiflora edulis.

Original languageEnglish (US)
Article number169
JournalViruses
Volume10
Issue number4
DOIs
StatePublished - Apr 1 2018

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Geminiviridae
Satellite Viruses
Brazil
Fruit
Viruses
Begomovirus
Citrus
Genome
Passiflora
Capsid Proteins
Virus Diseases
Arabidopsis
Tobacco

Keywords

  • Geminivirus
  • Passiflora sp
  • Passion fruit

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Fontenele, R. S., Abreu, R. A., Lamas, N. S., Alves-Freitas, D. M. T., Vidal, A. H., Poppiel, R. R., ... Ribeiro, S. G. (2018). Passion fruit chlorotic mottle virus: Molecular characterization of a new divergent geminivirus in Brazil. Viruses, 10(4), [169]. https://doi.org/10.3390/v10040169

Passion fruit chlorotic mottle virus : Molecular characterization of a new divergent geminivirus in Brazil. / Fontenele, Rafaela S.; Abreu, Rayane A.; Lamas, Natalia S.; Alves-Freitas, Dione M.T.; Vidal, Andreza H.; Poppiel, Raul R.; Melo, Fernando L.; Lacorte, Cristiano; Martin, Darren P.; Campos, Magnolia A.; Varsani, Arvind; Ribeiro, Simone G.

In: Viruses, Vol. 10, No. 4, 169, 01.04.2018.

Research output: Contribution to journalArticle

Fontenele, RS, Abreu, RA, Lamas, NS, Alves-Freitas, DMT, Vidal, AH, Poppiel, RR, Melo, FL, Lacorte, C, Martin, DP, Campos, MA, Varsani, A & Ribeiro, SG 2018, 'Passion fruit chlorotic mottle virus: Molecular characterization of a new divergent geminivirus in Brazil', Viruses, vol. 10, no. 4, 169. https://doi.org/10.3390/v10040169
Fontenele RS, Abreu RA, Lamas NS, Alves-Freitas DMT, Vidal AH, Poppiel RR et al. Passion fruit chlorotic mottle virus: Molecular characterization of a new divergent geminivirus in Brazil. Viruses. 2018 Apr 1;10(4). 169. https://doi.org/10.3390/v10040169
Fontenele, Rafaela S. ; Abreu, Rayane A. ; Lamas, Natalia S. ; Alves-Freitas, Dione M.T. ; Vidal, Andreza H. ; Poppiel, Raul R. ; Melo, Fernando L. ; Lacorte, Cristiano ; Martin, Darren P. ; Campos, Magnolia A. ; Varsani, Arvind ; Ribeiro, Simone G. / Passion fruit chlorotic mottle virus : Molecular characterization of a new divergent geminivirus in Brazil. In: Viruses. 2018 ; Vol. 10, No. 4.
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abstract = "Brazil is one of the major passion fruit producers worldwide. Viral diseases are among the most important constraints for passion fruit production. Here we identify and characterize a new passion fruit infecting-virus belonging to the family Geminiviridae: passion fruit chlorotic mottle virus (PCMoV). PCMoV is a divergent geminivirus unlike previously characterized passion fruit-infecting geminiviruses that belonged to the genus Begomovirus. Among the presently known geminiviruses, it is most closely related to, and shares ~62{\%} genome-wide identity with citrus chlorotic dwarf associated virus (CCDaV) and camelia chlorotic dwarf associated virus (CaCDaV). The 3743 nt PCMoV genome encodes a capsid protein (CP) and replication-associated protein (Rep) that respectively share 56 and 60{\%} amino acid identity with those encoded by CaCDaV. The CPs of PCMoV, CCDaV, and CaCDaV cluster with those of begomovirus whereas their Reps with those of becurtoviruses. Hence, these viruses likely represent a lineage of recombinant begomo-like and becurto-like ancestral viruses. Furthermore, PCMoV, CCDaV, and CaCDaV genomes are ~12–30{\%} larger than monopartite geminiviruses and this is primarily due to the encoded movement protein (MP; 891–921 nt) and this MP is most closely related to that encoded by the DNA-B component of bipartite begomoviruses. Hence, PCMoV, CCDaV, and CaCDaV lineage of viruses may represent molecules in an intermediary step in the evolution of bipartite begomoviruses (~5.3 kb) from monopartite geminiviruses (~2.7–3 kb). An infectious clone of PCMoV systemically infected Nicotiana benthamina, Arabidopsis thaliana, and Passiflora edulis.",
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