Osteopontin is required for the early onset of high fat diet-induced insulin resistance in mice

Justin Chapman, Philip D. Miles, Jachelle M. Ofrecio, Jaap G. Neels, Joseph G. Yu, Jamie L. Resnik, Jason Wilkes, Saswata Talukdar, Divya Thapar, Kristen Johnson, Dorothy D. Sears

Research output: Contribution to journalArticle

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Abstract

Background: Insulin resistance is manifested in muscle, adipose tissue, and liver and is associated with adipose tissue inflammation. The cellular components and mechanisms that regulate the onset of diet-induced insulin resistance are not clearly defined. Methodology and Principal Findings:We initially observed osteopontin (OPN) mRNA over-expression in adipose tissue of obese, insulin resistant humans and rats which was normalized by thiazolidinedione (TZD) treatment in both species. OPN regulates inflammation and is implicated in pathogenic maladies resulting from chronic obesity. Thus, we tested the hypothesis that OPN is involved in the early development of insulin resistance using a 2-4 week high fat diet (HFD) model. OPN KO mice fed HFD for 2 weeks were completely protected from the severe skeletal muscle, liver and adipose tissue insulin resistance that developed in wild type (WT) controls, as determined by hyperinsulinemic euglycemic clamp and acute insulin-stimulation studies. Although two-week HFD did not alter body weight or plasma free fatty acids and cytokines in either strain, HFD-induced hyperleptinemia, increased adipose tissue inflammation (macrophages and cytokines), and adipocyte hypertrophy were significant in WT mice and blunted or absent in OPN KO mice. Adipose tissue OPN protein isoform expression was significantly altered in 2- and 4-week HFD-fed WT mice but total OPN protein was unchanged. OPN KO bone marrow stromal cells were more osteogenic and less adipogenic than WT cells in vitro. Interestingly, the two differentiation pathways were inversely affected by HFD in WT cells in vitro. Conclusions:The OPN KO phenotypes we report reflect protection from insulin resistance that is associated with changes in adipocyte biology and adipose tissue inflammatory status. OPN is a key component in the development of HFD-induced insulin resistance.

Original languageEnglish (US)
Article numbere13959
JournalPloS one
Volume5
Issue number11
DOIs
StatePublished - Dec 9 2010
Externally publishedYes

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osteopontin
Osteopontin
High Fat Diet
high fat diet
Nutrition
insulin resistance
Insulin Resistance
Fats
Insulin
Adipose Tissue
adipose tissue
mice
Tissue
inflammation
Inflammation
Adipocytes
adipocytes
Liver
Muscle
cytokines

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Chapman, J., Miles, P. D., Ofrecio, J. M., Neels, J. G., Yu, J. G., Resnik, J. L., ... Sears, D. D. (2010). Osteopontin is required for the early onset of high fat diet-induced insulin resistance in mice. PloS one, 5(11), [e13959]. https://doi.org/10.1371/journal.pone.0013959

Osteopontin is required for the early onset of high fat diet-induced insulin resistance in mice. / Chapman, Justin; Miles, Philip D.; Ofrecio, Jachelle M.; Neels, Jaap G.; Yu, Joseph G.; Resnik, Jamie L.; Wilkes, Jason; Talukdar, Saswata; Thapar, Divya; Johnson, Kristen; Sears, Dorothy D.

In: PloS one, Vol. 5, No. 11, e13959, 09.12.2010.

Research output: Contribution to journalArticle

Chapman, J, Miles, PD, Ofrecio, JM, Neels, JG, Yu, JG, Resnik, JL, Wilkes, J, Talukdar, S, Thapar, D, Johnson, K & Sears, DD 2010, 'Osteopontin is required for the early onset of high fat diet-induced insulin resistance in mice', PloS one, vol. 5, no. 11, e13959. https://doi.org/10.1371/journal.pone.0013959
Chapman, Justin ; Miles, Philip D. ; Ofrecio, Jachelle M. ; Neels, Jaap G. ; Yu, Joseph G. ; Resnik, Jamie L. ; Wilkes, Jason ; Talukdar, Saswata ; Thapar, Divya ; Johnson, Kristen ; Sears, Dorothy D. / Osteopontin is required for the early onset of high fat diet-induced insulin resistance in mice. In: PloS one. 2010 ; Vol. 5, No. 11.
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AU - Chapman, Justin

AU - Miles, Philip D.

AU - Ofrecio, Jachelle M.

AU - Neels, Jaap G.

AU - Yu, Joseph G.

AU - Resnik, Jamie L.

AU - Wilkes, Jason

AU - Talukdar, Saswata

AU - Thapar, Divya

AU - Johnson, Kristen

AU - Sears, Dorothy D.

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N2 - Background: Insulin resistance is manifested in muscle, adipose tissue, and liver and is associated with adipose tissue inflammation. The cellular components and mechanisms that regulate the onset of diet-induced insulin resistance are not clearly defined. Methodology and Principal Findings:We initially observed osteopontin (OPN) mRNA over-expression in adipose tissue of obese, insulin resistant humans and rats which was normalized by thiazolidinedione (TZD) treatment in both species. OPN regulates inflammation and is implicated in pathogenic maladies resulting from chronic obesity. Thus, we tested the hypothesis that OPN is involved in the early development of insulin resistance using a 2-4 week high fat diet (HFD) model. OPN KO mice fed HFD for 2 weeks were completely protected from the severe skeletal muscle, liver and adipose tissue insulin resistance that developed in wild type (WT) controls, as determined by hyperinsulinemic euglycemic clamp and acute insulin-stimulation studies. Although two-week HFD did not alter body weight or plasma free fatty acids and cytokines in either strain, HFD-induced hyperleptinemia, increased adipose tissue inflammation (macrophages and cytokines), and adipocyte hypertrophy were significant in WT mice and blunted or absent in OPN KO mice. Adipose tissue OPN protein isoform expression was significantly altered in 2- and 4-week HFD-fed WT mice but total OPN protein was unchanged. OPN KO bone marrow stromal cells were more osteogenic and less adipogenic than WT cells in vitro. Interestingly, the two differentiation pathways were inversely affected by HFD in WT cells in vitro. Conclusions:The OPN KO phenotypes we report reflect protection from insulin resistance that is associated with changes in adipocyte biology and adipose tissue inflammatory status. OPN is a key component in the development of HFD-induced insulin resistance.

AB - Background: Insulin resistance is manifested in muscle, adipose tissue, and liver and is associated with adipose tissue inflammation. The cellular components and mechanisms that regulate the onset of diet-induced insulin resistance are not clearly defined. Methodology and Principal Findings:We initially observed osteopontin (OPN) mRNA over-expression in adipose tissue of obese, insulin resistant humans and rats which was normalized by thiazolidinedione (TZD) treatment in both species. OPN regulates inflammation and is implicated in pathogenic maladies resulting from chronic obesity. Thus, we tested the hypothesis that OPN is involved in the early development of insulin resistance using a 2-4 week high fat diet (HFD) model. OPN KO mice fed HFD for 2 weeks were completely protected from the severe skeletal muscle, liver and adipose tissue insulin resistance that developed in wild type (WT) controls, as determined by hyperinsulinemic euglycemic clamp and acute insulin-stimulation studies. Although two-week HFD did not alter body weight or plasma free fatty acids and cytokines in either strain, HFD-induced hyperleptinemia, increased adipose tissue inflammation (macrophages and cytokines), and adipocyte hypertrophy were significant in WT mice and blunted or absent in OPN KO mice. Adipose tissue OPN protein isoform expression was significantly altered in 2- and 4-week HFD-fed WT mice but total OPN protein was unchanged. OPN KO bone marrow stromal cells were more osteogenic and less adipogenic than WT cells in vitro. Interestingly, the two differentiation pathways were inversely affected by HFD in WT cells in vitro. Conclusions:The OPN KO phenotypes we report reflect protection from insulin resistance that is associated with changes in adipocyte biology and adipose tissue inflammatory status. OPN is a key component in the development of HFD-induced insulin resistance.

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