TY - JOUR
T1 - Organic Hyperbolic Material Assisted Illumination Nanoscopy
AU - Lee, Yeon Ui
AU - Posner, Clara
AU - Nie, Zhaoyu
AU - Zhao, Junxiang
AU - Li, Shilong
AU - Bopp, Steven Edward
AU - Wisna, Gde Bimananda Mahardika
AU - Ha, Jeongho
AU - Song, Chengyu
AU - Zhang, Jin
AU - Yang, Sui
AU - Zhang, Xiang
AU - Liu, Zhaowei
N1 - Funding Information:
This work was supported by the Gordon and Betty Moore Foundation (to Z.L.), the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2021R1F1A1062916), the research grant of the Chungbuk National University in 2021 (to Y.U.L.). G.B.M.W. acknowledges two years scholarship from LPDP (Indonesia Endowment Fund for Education) for pursuing master degree in United States. C.P. was supported by the Molecular Biophysics Training Grant, NIH Grant T32 GM008326.
Publisher Copyright:
© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH
PY - 2021/11/17
Y1 - 2021/11/17
N2 - Resolution capability of the linear structured illumination microscopy (SIM) plays a key role in its applications in physics, medicine, biology, and life science. Many advanced methodologies have been developed to extend the resolution of structured illumination by using subdiffraction-limited optical excitation patterns. However, obtaining SIM images with a resolution beyond 40 nm at visible frequency remains as an insurmountable obstacle due to the intrinsic limitation of spatial frequency bandwidth of the involved materials and the complexity of the illumination system. Here, a low-loss natural organic hyperbolic material (OHM) that can support record high spatial-frequency modes beyond 50k0, i.e., effective refractive index larger than 50, at visible frequencies is reported. OHM-based speckle structured illumination microscopy demonstrates imaging resolution at 30 nm scales with enhanced fluorophore photostability, biocompatibility, easy to use and low cost. This study will open up a new route in super-resolution microscopy by utilizing OHM films for various applications including bioimaging and sensing.
AB - Resolution capability of the linear structured illumination microscopy (SIM) plays a key role in its applications in physics, medicine, biology, and life science. Many advanced methodologies have been developed to extend the resolution of structured illumination by using subdiffraction-limited optical excitation patterns. However, obtaining SIM images with a resolution beyond 40 nm at visible frequency remains as an insurmountable obstacle due to the intrinsic limitation of spatial frequency bandwidth of the involved materials and the complexity of the illumination system. Here, a low-loss natural organic hyperbolic material (OHM) that can support record high spatial-frequency modes beyond 50k0, i.e., effective refractive index larger than 50, at visible frequencies is reported. OHM-based speckle structured illumination microscopy demonstrates imaging resolution at 30 nm scales with enhanced fluorophore photostability, biocompatibility, easy to use and low cost. This study will open up a new route in super-resolution microscopy by utilizing OHM films for various applications including bioimaging and sensing.
KW - bioimaging
KW - organic hyperbolic materials
KW - poly(3-hexylthiophenes)
KW - structured illumination microscopy
KW - super-resolution microscopy
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U2 - 10.1002/advs.202102230
DO - 10.1002/advs.202102230
M3 - Article
C2 - 34436815
AN - SCOPUS:85113391181
SN - 2198-3844
VL - 8
JO - Advanced Science
JF - Advanced Science
IS - 22
M1 - 2102230
ER -