Open data set of live cyanobacterial cells imaged using an X-ray laser

Gijs Van Der Schot; Martin Svenda; Filipe R N C Maia; Max F. Hantke; Daniel P. DePonte; M. Marvin Seibert; Andrew Aquila; Joachim Schulz; Richard Kirian; Mengning Liang; Francesco Stellato; Sadia Bari; Bianca Iwan; Jakob Andreasson; Nicusor Timneanu; Johan Bielecki; Daniel Westphal; Francisca Nunes De Almeida; Dusko Odic; Dirk Hasse; Gunilla H. Carlsson; Daniel S D Larsson; Anton Barty; Andrew V. Martin; Sebastian Schorb; Christoph Bostedt; John D. Bozek; Sebastian Carron; Ken Ferguson; Daniel Rolles; Artem Rudenko; Sascha W. Epp; Lutz Foucar; Benedikt Rudek; Benjamin Erk; Robert Hartmann; Nils Kimmel; Peter Holl; Lars Englert; N. Duane Loh; Henry N. Chapman; Inger Andersson; Janos Hajdu; Tomas Ekeberg

doi:10.1038/sdata.2016.58

Open data set of live cyanobacterial cells imaged using an X-ray laser

Gijs Van Der Schot, Martin Svenda, Filipe R N C Maia, Max F. Hantke, Daniel P. DePonte, M. Marvin Seibert, Andrew Aquila, Joachim Schulz, Richard Kirian, Mengning Liang, Francesco Stellato, Sadia Bari, Bianca Iwan, Jakob Andreasson, Nicusor Timneanu, Johan Bielecki, Daniel Westphal, Francisca Nunes De Almeida, Dusko Odic, Dirk HasseGunilla H. Carlsson, Daniel S D Larsson, Anton Barty, Andrew V. Martin, Sebastian Schorb, Christoph Bostedt, John D. Bozek, Sebastian Carron, Ken Ferguson, Daniel Rolles, Artem Rudenko, Sascha W. Epp, Lutz Foucar, Benedikt Rudek, Benjamin Erk, Robert Hartmann, Nils Kimmel, Peter Holl, Lars Englert, N. Duane Loh, Henry N. Chapman, Inger Andersson, Janos Hajdu, Tomas Ekeberg

Research output: Contribution to journal › Article › peer-review

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Abstract

Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and structural variability in large populations of cells and to create abstract models. Such studies will allow us to understand living cells and populations of cells in new ways. New X-ray lasers, like the European XFEL, will produce billions of pulses per day, and could open new areas in structural sciences.

Original language	English (US)
Article number	160058
Journal	Scientific Data
Volume	3
DOIs	https://doi.org/10.1038/sdata.2016.58
State	Published - Aug 1 2016

ASJC Scopus subject areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

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10.1038/sdata.2016.58

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Van Der Schot, G., Svenda, M., Maia, F. R. N. C., Hantke, M. F., DePonte, D. P., Seibert, M. M., Aquila, A., Schulz, J., Kirian, R., Liang, M., Stellato, F., Bari, S., Iwan, B., Andreasson, J., Timneanu, N., Bielecki, J., Westphal, D., Nunes De Almeida, F., Odic, D., ... Ekeberg, T. (2016). Open data set of live cyanobacterial cells imaged using an X-ray laser. Scientific Data, 3, Article 160058. https://doi.org/10.1038/sdata.2016.58

Van Der Schot, G, Svenda, M, Maia, FRNC, Hantke, MF, DePonte, DP, Seibert, MM, Aquila, A, Schulz, J, Kirian, R, Liang, M, Stellato, F, Bari, S, Iwan, B, Andreasson, J, Timneanu, N, Bielecki, J, Westphal, D, Nunes De Almeida, F, Odic, D, Hasse, D, Carlsson, GH, Larsson, DSD, Barty, A, Martin, AV, Schorb, S, Bostedt, C, Bozek, JD, Carron, S, Ferguson, K, Rolles, D, Rudenko, A, Epp, SW, Foucar, L, Rudek, B, Erk, B, Hartmann, R, Kimmel, N, Holl, P, Englert, L, Loh, ND, Chapman, HN, Andersson, I, Hajdu, J & Ekeberg, T 2016, 'Open data set of live cyanobacterial cells imaged using an X-ray laser', Scientific Data, vol. 3, 160058. https://doi.org/10.1038/sdata.2016.58

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title = "Open data set of live cyanobacterial cells imaged using an X-ray laser",

abstract = "Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and structural variability in large populations of cells and to create abstract models. Such studies will allow us to understand living cells and populations of cells in new ways. New X-ray lasers, like the European XFEL, will produce billions of pulses per day, and could open new areas in structural sciences.",

author = "{Van Der Schot}, Gijs and Martin Svenda and Maia, {Filipe R N C} and Hantke, {Max F.} and DePonte, {Daniel P.} and Seibert, {M. Marvin} and Andrew Aquila and Joachim Schulz and Richard Kirian and Mengning Liang and Francesco Stellato and Sadia Bari and Bianca Iwan and Jakob Andreasson and Nicusor Timneanu and Johan Bielecki and Daniel Westphal and {Nunes De Almeida}, Francisca and Dusko Odic and Dirk Hasse and Carlsson, {Gunilla H.} and Larsson, {Daniel S D} and Anton Barty and Martin, {Andrew V.} and Sebastian Schorb and Christoph Bostedt and Bozek, {John D.} and Sebastian Carron and Ken Ferguson and Daniel Rolles and Artem Rudenko and Epp, {Sascha W.} and Lutz Foucar and Benedikt Rudek and Benjamin Erk and Robert Hartmann and Nils Kimmel and Peter Holl and Lars Englert and Loh, {N. Duane} and Chapman, {Henry N.} and Inger Andersson and Janos Hajdu and Tomas Ekeberg",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2016.",

year = "2016",

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doi = "10.1038/sdata.2016.58",

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T1 - Open data set of live cyanobacterial cells imaged using an X-ray laser

AU - Van Der Schot, Gijs

AU - Svenda, Martin

AU - Maia, Filipe R N C

AU - Hantke, Max F.

AU - DePonte, Daniel P.

AU - Seibert, M. Marvin

AU - Aquila, Andrew

AU - Schulz, Joachim

AU - Kirian, Richard

AU - Liang, Mengning

AU - Stellato, Francesco

AU - Bari, Sadia

AU - Iwan, Bianca

AU - Andreasson, Jakob

AU - Timneanu, Nicusor

AU - Bielecki, Johan

AU - Westphal, Daniel

AU - Nunes De Almeida, Francisca

AU - Odic, Dusko

AU - Hasse, Dirk

AU - Carlsson, Gunilla H.

AU - Larsson, Daniel S D

AU - Barty, Anton

AU - Martin, Andrew V.

AU - Schorb, Sebastian

AU - Bostedt, Christoph

AU - Bozek, John D.

AU - Carron, Sebastian

AU - Ferguson, Ken

AU - Rolles, Daniel

AU - Rudenko, Artem

AU - Epp, Sascha W.

AU - Foucar, Lutz

AU - Rudek, Benedikt

AU - Erk, Benjamin

AU - Hartmann, Robert

AU - Kimmel, Nils

AU - Holl, Peter

AU - Englert, Lars

AU - Loh, N. Duane

AU - Chapman, Henry N.

AU - Andersson, Inger

AU - Hajdu, Janos

AU - Ekeberg, Tomas

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and structural variability in large populations of cells and to create abstract models. Such studies will allow us to understand living cells and populations of cells in new ways. New X-ray lasers, like the European XFEL, will produce billions of pulses per day, and could open new areas in structural sciences.

AB - Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and structural variability in large populations of cells and to create abstract models. Such studies will allow us to understand living cells and populations of cells in new ways. New X-ray lasers, like the European XFEL, will produce billions of pulses per day, and could open new areas in structural sciences.

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ER -

Open data set of live cyanobacterial cells imaged using an X-ray laser

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