Nondissociation of GAL4 and GAL80 in vivo after galactose induction

Kerstin K. Leuther; Stephen A. Johnston

doi:10.1126/science.1598579

Nondissociation of GAL4 and GAL80 in vivo after galactose induction

Kerstin K. Leuther, Stephen A. Johnston

Research output: Contribution to journal › Article › peer-review

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Abstract

Transcription of galactose-inducible genes in yeast is regulated by interaction between the activator protein GAL4 and the negative regulatory protein GAL80. It has been suggested that GAL80 binds to and represses GAL4 under uninduced conditions and dissociates from GAL4 on induction. However, the possibility that GAL80 remains associated with GAL4 after induction has not been ruled out. Experiments to discriminate between these two models were performed and revealed that GAL80 stays bound after induction.

Original language	English (US)
Pages (from-to)	1333-1335
Number of pages	3
Journal	Science
Volume	256
Issue number	5064
DOIs	https://doi.org/10.1126/science.1598579
State	Published - 1992
Externally published	Yes

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title = "Nondissociation of GAL4 and GAL80 in vivo after galactose induction",

abstract = "Transcription of galactose-inducible genes in yeast is regulated by interaction between the activator protein GAL4 and the negative regulatory protein GAL80. It has been suggested that GAL80 binds to and represses GAL4 under uninduced conditions and dissociates from GAL4 on induction. However, the possibility that GAL80 remains associated with GAL4 after induction has not been ruled out. Experiments to discriminate between these two models were performed and revealed that GAL80 stays bound after induction.",

author = "Leuther, {Kerstin K.} and Johnston, {Stephen A.}",

year = "1992",

doi = "10.1126/science.1598579",

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T1 - Nondissociation of GAL4 and GAL80 in vivo after galactose induction

AU - Leuther, Kerstin K.

AU - Johnston, Stephen A.

PY - 1992

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AB - Transcription of galactose-inducible genes in yeast is regulated by interaction between the activator protein GAL4 and the negative regulatory protein GAL80. It has been suggested that GAL80 binds to and represses GAL4 under uninduced conditions and dissociates from GAL4 on induction. However, the possibility that GAL80 remains associated with GAL4 after induction has not been ruled out. Experiments to discriminate between these two models were performed and revealed that GAL80 stays bound after induction.

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JF - Science

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ER -

Nondissociation of GAL4 and GAL80 in vivo after galactose induction

Abstract

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