Neurosecretory cell-based biosensor: Monitoring secretion of adrenal chromaffin cells by local extracellular acidification using light-addressable potentiometric sensor

Qingjun Liu, Ning Hu, Fenni Zhang, Hua Wang, Weiwei Ye, Ping Wang

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Vesicular exocytosis plays an important role in many physiological processes. The dense-core vesicles release of chromaffi{ligature}n cells is a suitable model for the presynaptic process in neurosecretory cells. In this study, light-addressable potentiometric sensor (LAPS) was introduced as a label-free recording method for vesicle release by the local extracellular acidification. The chromaffi{ligature}n cells are directly cultured on the sensor surface. After cells and LAPS hybrid system is established, the events of vesicular exocytosis are recorded. Protons stored in the vesicles and co-released with transmitters, induced a brief acidic shifts in the cell-sensor cleft. Under the stimulation of the KCl and acetylcholine (Ach), the signals presented the different amplitude and exocytosis rate, and reflected the specific features of the exocytosis. The result indicates that neurosecretory cell-based biosensor will provide a useful platform for neurosecretion mechanism research by monitoring the exocytotic activities with extracellular acidification sensing.

Original languageEnglish (US)
Pages (from-to)421-424
Number of pages4
JournalBiosensors and Bioelectronics
Volume35
Issue number1
DOIs
StatePublished - May 15 2012
Externally publishedYes

Keywords

  • Cell-based biosensor
  • Chromaffin cell
  • Exocytosis
  • Light-addressable potentiometric sensor (LAPS)
  • Neurosecretory cell

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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