NADP regulates the yeast GAL induction system

P. Rajesh Kumar, Yao Yu, Rolf Sternglanz, Stephen Johnston, Leemor Joshua-Tor

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5′-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

Original languageEnglish (US)
Pages (from-to)1090-1092
Number of pages3
JournalScience
Volume319
Issue number5866
DOIs
StatePublished - Feb 22 2008

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Galactose
NADP
Yeasts
Transducers
Saccharomyces cerevisiae
Cytoplasm
Adenosine Triphosphate
Peptides
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kumar, P. R., Yu, Y., Sternglanz, R., Johnston, S., & Joshua-Tor, L. (2008). NADP regulates the yeast GAL induction system. Science, 319(5866), 1090-1092. https://doi.org/10.1126/science.1151903

NADP regulates the yeast GAL induction system. / Kumar, P. Rajesh; Yu, Yao; Sternglanz, Rolf; Johnston, Stephen; Joshua-Tor, Leemor.

In: Science, Vol. 319, No. 5866, 22.02.2008, p. 1090-1092.

Research output: Contribution to journalArticle

Kumar, PR, Yu, Y, Sternglanz, R, Johnston, S & Joshua-Tor, L 2008, 'NADP regulates the yeast GAL induction system', Science, vol. 319, no. 5866, pp. 1090-1092. https://doi.org/10.1126/science.1151903
Kumar PR, Yu Y, Sternglanz R, Johnston S, Joshua-Tor L. NADP regulates the yeast GAL induction system. Science. 2008 Feb 22;319(5866):1090-1092. https://doi.org/10.1126/science.1151903
Kumar, P. Rajesh ; Yu, Yao ; Sternglanz, Rolf ; Johnston, Stephen ; Joshua-Tor, Leemor. / NADP regulates the yeast GAL induction system. In: Science. 2008 ; Vol. 319, No. 5866. pp. 1090-1092.
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