NADP regulates the yeast GAL induction system

P. Rajesh Kumar, Yao Yu, Rolf Sternglanz, Stephen Johnston, Leemor Joshua-Tor

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5′-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

Original languageEnglish (US)
Pages (from-to)1090-1092
Number of pages3
JournalScience
Volume319
Issue number5866
DOIs
StatePublished - Feb 22 2008

ASJC Scopus subject areas

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    Kumar, P. R., Yu, Y., Sternglanz, R., Johnston, S., & Joshua-Tor, L. (2008). NADP regulates the yeast GAL induction system. Science, 319(5866), 1090-1092. https://doi.org/10.1126/science.1151903