Abstract
Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5′-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1090-1092 |
| Number of pages | 3 |
| Journal | Science |
| Volume | 319 |
| Issue number | 5866 |
| DOIs | |
| State | Published - Feb 22 2008 |
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Cite this
NADP regulates the yeast GAL induction system. / Kumar, P. Rajesh; Yu, Yao; Sternglanz, Rolf; Johnston, Stephen; Joshua-Tor, Leemor.
In: Science, Vol. 319, No. 5866, 22.02.2008, p. 1090-1092.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - NADP regulates the yeast GAL induction system
AU - Kumar, P. Rajesh
AU - Yu, Yao
AU - Sternglanz, Rolf
AU - Johnston, Stephen
AU - Joshua-Tor, Leemor
PY - 2008/2/22
Y1 - 2008/2/22
N2 - Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5′-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
AB - Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5′-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
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UR - http://www.scopus.com/inward/citedby.url?scp=39749150952&partnerID=8YFLogxK
U2 - 10.1126/science.1151903
DO - 10.1126/science.1151903
M3 - Article
C2 - 18292341
AN - SCOPUS:39749150952
VL - 319
SP - 1090
EP - 1092
JO - Science
JF - Science
SN - 0036-8075
IS - 5866
ER -