Abstract
Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5′-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
Original language | English (US) |
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Pages (from-to) | 1090-1092 |
Number of pages | 3 |
Journal | Science |
Volume | 319 |
Issue number | 5866 |
DOIs | |
State | Published - Feb 22 2008 |
ASJC Scopus subject areas
- General
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Dive into the research topics of 'NADP regulates the yeast GAL induction system'. Together they form a unique fingerprint.Datasets
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Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S0)-[G301R]
Kumar, P. R. (Contributor), Yu, Y. (Contributor), Sternglanz, R. (Contributor), Johnston, S. (Contributor) & Joshua-Tor, L. (Contributor), Protein Data Bank (PDB), Mar 4 2008
DOI: 10.2210/pdb3BTV, https://www.wwpdb.org/pdb?id=pdb_00003btv
Dataset
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Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD
Kumar, P. R. (Contributor), Yu, Y. (Contributor), Sternglanz, R. (Contributor), Johnston, S. (Contributor) & Joshua-Tor, L. (Contributor), Protein Data Bank (PDB), Mar 4 2008
DOI: 10.2210/pdb3BTS, https://www.wwpdb.org/pdb?id=pdb_00003bts
Dataset
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Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S2) [E351K]
Kumar, P. R. (Contributor), Yu, Y. (Contributor), Sternglanz, R. (Contributor), Johnston, S. (Contributor) & Joshua-Tor, L. (Contributor), Protein Data Bank (PDB), Mar 4 2008
DOI: 10.2210/pdb3BTU, https://www.wwpdb.org/pdb?id=pdb_00003btu
Dataset