Muscle-Specific Sensitivity to Voluntary Physical Activity and Detraining

Jon Philippe K. Hyatt, Emily A. Brown, Hannah M. Deacon, Gary E. McCall

Research output: Contribution to journalArticle

Abstract

Aerobic physical activity triggers adaptations in skeletal muscle including a fast-to-slow shift in myosin heavy chain (MHC) isoforms, an enhanced capillary network, and mitochondrial biogenesis to meet increased demands placed upon this tissue. Although the magnitude of these responses appears to be dependent on muscle phenotype as well as training volume and/or intensity, the whole-muscle response to detraining remains mostly unexplored. Here, we hypothesized that the shifts toward slower MHC phentotype and the increased capillarity and mitochondrial oxidative markers induced with training would return toward sedentary (SED) control levels sooner in the fast plantaris than in the slow soleus muscle as a result of detraining. Soleus and plantaris muscles from 8-week (TR 8wk) voluntarily running adult female Sprague–Dawley rats were compared to muscles from SED and detrained rats (DETR) (4 weeks voluntary running followed by 4 weeks of reduced activity), which were subdivided into low- (DETR Lo) and high-running-distance (DETR Hi) groups. We show that maintaining the fast-to-slow MHC isoform shift required consistent aerobic training in the soleus and plantaris muscles: detraining clearly abolished any fast-to-slow gains in the plantaris, whereas the training volume in DETR Hi rats appeared to influence the MHC return to basal levels in the soleus. Total capillary number (per mm2) in the plantaris increased in all groups compared to SED levels, but, in the soleus, this enhancement was observed only in the TR 8wk rats. Generally, increased mitochondrial markers for aerobicitiy were observed in TR 8wk plantaris, but not soleus, muscles. In a second experiment, we show that the muscle-specific adaptations were similar after 4 weeks of voluntary exercise (TR 4wk) as in 4 weeks (TR 8wk). Taken together, our findings suggest that the plantaris muscle is more sensitive to voluntary physical activity and detraining than the soleus muscle; these results also demonstrate that the soleus muscle requires a greater aerobic challenge (i.e., intensity, duration) to trigger phenotypic, angiogenic, or aerobic enzyme adaptations. Our findings generally suggest that muscular aerobic fitness to voluntary running, or its loss during detraining, manifests as changes occurring primarily within fast, rather than slow, muscle phenotypes.

Original languageEnglish (US)
Article number1328
JournalFrontiers in Physiology
Volume10
DOIs
StatePublished - Oct 23 2019
Externally publishedYes

Fingerprint

Skeletal Muscle
Muscles
Myosin Heavy Chains
Running
Protein Isoforms
Capillary Action
Phenotype
Organelle Biogenesis
Enzymes

Keywords

  • capillary
  • citrate synthase
  • myosin heavy chain
  • OXPHOS
  • plantaris
  • rat
  • soleus

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Muscle-Specific Sensitivity to Voluntary Physical Activity and Detraining. / Hyatt, Jon Philippe K.; Brown, Emily A.; Deacon, Hannah M.; McCall, Gary E.

In: Frontiers in Physiology, Vol. 10, 1328, 23.10.2019.

Research output: Contribution to journalArticle

Hyatt, Jon Philippe K. ; Brown, Emily A. ; Deacon, Hannah M. ; McCall, Gary E. / Muscle-Specific Sensitivity to Voluntary Physical Activity and Detraining. In: Frontiers in Physiology. 2019 ; Vol. 10.
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