TY - JOUR
T1 - mRNA usage during Drosophila melanogaster embryonic development. Analysis of nine cloned DNA segments
AU - Brogan, Kelle L.
AU - Goldstein, Elliott S.
N1 - Funding Information:
We would like to thank Drs Winifred Doane, Edward Birge, Robert Gemmill, and Walter Vincent for their advice and criticism, and Dr Norman Davidson for the gift of the Drosophila Lambda library. This project was begun in the laboratory of Dr Eliot Myerowitz and we are grateful for his help. This research was supported in part by NIH grant no. HDO9157 and was done in partial fulfillment of the requirements for the degree of Master of Science by K. L. B.
PY - 1985/5
Y1 - 1985/5
N2 - A set of nine phage lambda clones containing inserts from Drosophila melanogaster which are complementary to cDNA made from oocyte poly(A)+ RNA were selected from a larger group. These cloned elements code for a range of middle abundant RNA sequences which show no appreciable change in abundance during Drosophila embryogenesis. Seven of the nine clones are complementary to two oocyte RNAs, one to three RNAs and one to four RNAs. This study describes the changes that occur in these RNAs during embryonic development in the polysomal and non-polysomal fraction, and in the poly(A)+ RNA and poly(A)- RNA fraction. In all nine of these clones, greater than 70% of the complementary RNA is found in the polysomal region of a sucrose gradient. This proportion increases somewhat during development. Specific changes have been found during development in the proportion of RNA that is poly(A)+. Depending to the cloned sequence, this proportion may increase, decrease, or remain unchanged. For those clones that show a change, most of this change occurs between 8 and 19 h of development. Our data suggest, furthermore, the presence of a class of non-adenylated RNA being utilized during embryogenesis.
AB - A set of nine phage lambda clones containing inserts from Drosophila melanogaster which are complementary to cDNA made from oocyte poly(A)+ RNA were selected from a larger group. These cloned elements code for a range of middle abundant RNA sequences which show no appreciable change in abundance during Drosophila embryogenesis. Seven of the nine clones are complementary to two oocyte RNAs, one to three RNAs and one to four RNAs. This study describes the changes that occur in these RNAs during embryonic development in the polysomal and non-polysomal fraction, and in the poly(A)+ RNA and poly(A)- RNA fraction. In all nine of these clones, greater than 70% of the complementary RNA is found in the polysomal region of a sucrose gradient. This proportion increases somewhat during development. Specific changes have been found during development in the proportion of RNA that is poly(A)+. Depending to the cloned sequence, this proportion may increase, decrease, or remain unchanged. For those clones that show a change, most of this change occurs between 8 and 19 h of development. Our data suggest, furthermore, the presence of a class of non-adenylated RNA being utilized during embryogenesis.
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U2 - 10.1016/0014-4827(85)90434-3
DO - 10.1016/0014-4827(85)90434-3
M3 - Article
C2 - 2581797
AN - SCOPUS:0021875411
SN - 0014-4827
VL - 158
SP - 95
EP - 103
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -