Morphometric identification of queens, workers, intermediates in in vitro reared honey bees (Apis mellifera)

Daiana A. De Souza, Ying Wang, Osman Kaftanoglu, David De Jong, Gro Amdam, Lionel S. Gonçalves, Tiago M. Francoy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In vitro rearing is an important and useful tool for honey bee (Apis mellifera L.) studies. However, it often results in intercastes between queens and workers, which are normally are not seen in hive-reared bees, except when larvae older than three days are grafted for queen rearing. Morphological classification (queen versus worker or intercastes) of bees produced by this method can be subjective and generally depends on size differences. Here, we propose an alternative method for caste classification of female honey bees reared in vitro, based on weight at emergence, ovariole number, spermatheca size and size and shape, and features of the head, mandible and basitarsus. Morphological measurements were made with both traditional morphometric and geometric morphometrics techniques. The classifications were performed by principal component analysis, using naturally developed queens and workers as controls. First, the analysis included all the characters. Subsequently, a new analysis was made without the information about ovariole number and spermatheca size. Geometric morphometrics was less dependent on ovariole number and spermatheca information for caste and intercaste identification. This is useful, since acquiring information concerning these reproductive structures requires time-consuming dissection and they are not accessible when abdomens have been removed for molecular assays or in dried specimens. Additionally, geometric morphometrics divided intercastes into more discrete phenotype subsets. We conclude that morphometric geometrics are superior to traditional morphometrics techniques for identification and classification of honey bee castes and intermediates.

Original languageEnglish (US)
Article numbere0123663
JournalPLoS One
Volume10
Issue number4
DOIs
StatePublished - Apr 20 2015

Fingerprint

Honey
Bees
queen insects
Apis mellifera
ovarioles
spermatheca
honey bees
Social Class
queen rearing
beehives
Dissection
methodology
abdomen
Principal component analysis
eclosion
Apoidea
Assays
principal component analysis
rearing
Urticaria

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

De Souza, D. A., Wang, Y., Kaftanoglu, O., De Jong, D., Amdam, G., Gonçalves, L. S., & Francoy, T. M. (2015). Morphometric identification of queens, workers, intermediates in in vitro reared honey bees (Apis mellifera). PLoS One, 10(4), [e0123663]. https://doi.org/10.1371/journal.pone.0123663

Morphometric identification of queens, workers, intermediates in in vitro reared honey bees (Apis mellifera). / De Souza, Daiana A.; Wang, Ying; Kaftanoglu, Osman; De Jong, David; Amdam, Gro; Gonçalves, Lionel S.; Francoy, Tiago M.

In: PLoS One, Vol. 10, No. 4, e0123663, 20.04.2015.

Research output: Contribution to journalArticle

De Souza, DA, Wang, Y, Kaftanoglu, O, De Jong, D, Amdam, G, Gonçalves, LS & Francoy, TM 2015, 'Morphometric identification of queens, workers, intermediates in in vitro reared honey bees (Apis mellifera)', PLoS One, vol. 10, no. 4, e0123663. https://doi.org/10.1371/journal.pone.0123663
De Souza, Daiana A. ; Wang, Ying ; Kaftanoglu, Osman ; De Jong, David ; Amdam, Gro ; Gonçalves, Lionel S. ; Francoy, Tiago M. / Morphometric identification of queens, workers, intermediates in in vitro reared honey bees (Apis mellifera). In: PLoS One. 2015 ; Vol. 10, No. 4.
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