Molecular structure, localization and function of biliproteins in the chlorophyll a/d containing oxygenic photosynthetic prokaryote Acaryochloris marina

Qiang Hu, Jürgen Marquardt, Ikuko Iwasaki, Hideaki Miyashita, Norihide Kurano, Erhard Mörschel, Shigetoh Miyachi

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

We investigated the localization, structure and function of the biliproteins of the oxygenic photosynthetic prokaryote Acaryochloris marina, the sole organism known to date that contains chlorophyll d as the predominant photosynthetic pigment. The biliproteins were isolated by means of sucrose gradient centrifugation, ion exchange and gel filtration chromatography. Up to six biliprotein subunits in a molecular mass range of 15.5-18.4 kDa were found that cross-reacted with antibodies raised against phycocyanin or allophycocyanin from a red alga. N-Terminal sequences of the α- and β-subunits of phycocyanin showed high homogeneity to those of cyanobacteria and red algae, but not to those of cryptomonads. As shown by electron microscopy, the native biliprotein aggregates are organized as rod-shaped structures and located on the cytoplasmic side of the thylakoid membranes predominantly in unstacked thylakoid regions. Biochemical and spectroscopic analysis revealed that they consist of four hexameric units, some of which are composed of phycocyanin alone, others of phycocyanin together with allophycocyanin. Spectroscopic analysis of isolated photosynthetic reaction center complexes demonstrated that the biliproteins are physically attached to the photosystem II complexes, transferring light energy to the photosystem II reaction center chlorophyll d with high efficiency. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)250-261
Number of pages12
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1412
Issue number3
DOIs
StatePublished - Aug 4 1999

Keywords

  • Acaryochloris marina
  • Chlorophyll d
  • Energy transfer
  • Photosystem II-biliprotein complex
  • Phycobiliprotein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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