Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta)

Sreetharan Kanthaswamy, Andrea Von Dollen, Jennifer D. Kurushima, Ona Alminas, Jeffrey Rogers, Betsy Ferguson, Nicholas W. Lerche, Philip C. Allen, David Glenn Smith

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

To preserve genetic variability and minimize genetic subdivision among captive Macaca, mulatta at each of the U.S. National Institutes of Health (NIH)-sponsored regional research colonies, the genetic structure of each colony must be characterized. To compare population genetic and demographic parameters across colonies and generations, one standard panel of highly informative genetic markers is required. We assembled a core marker set of four multiplex polymerase chain reaction (PCR) panels comprising 15 autosomal short tandem repeat (STR) loci with high information content selected from existing panels of well-characterized markers that are currently used for parentage assessment and genetic management of rhesus macaques. We then assessed the effectiveness of these loci for providing high probabilities of individual identification and parentage resolution, and for estimating population genetic parameters that are useful for genetic management.

Original languageEnglish (US)
Pages (from-to)73-95
Number of pages23
JournalAmerican Journal of Primatology
Volume68
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Fingerprint

Macaca mulatta
microsatellite repeats
genetic markers
parentage
population genetics
National Institutes of Health
loci
genetic marker
polymerase chain reaction
genetic structure
preserves
demographic statistics
genetic variation
marker
parameter

Keywords

  • Genetic diversity
  • Heterozygosity
  • Polymorphic information content (PIC)
  • Probability of genetic identity (PI)
  • Probability of parentage exclusion (PE)
  • Short tandem repeats (STRs)

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta). / Kanthaswamy, Sreetharan; Von Dollen, Andrea; Kurushima, Jennifer D.; Alminas, Ona; Rogers, Jeffrey; Ferguson, Betsy; Lerche, Nicholas W.; Allen, Philip C.; Smith, David Glenn.

In: American Journal of Primatology, Vol. 68, No. 1, 01.2006, p. 73-95.

Research output: Contribution to journalArticle

Kanthaswamy, S, Von Dollen, A, Kurushima, JD, Alminas, O, Rogers, J, Ferguson, B, Lerche, NW, Allen, PC & Smith, DG 2006, 'Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta)', American Journal of Primatology, vol. 68, no. 1, pp. 73-95. https://doi.org/10.1002/ajp.20207
Kanthaswamy, Sreetharan ; Von Dollen, Andrea ; Kurushima, Jennifer D. ; Alminas, Ona ; Rogers, Jeffrey ; Ferguson, Betsy ; Lerche, Nicholas W. ; Allen, Philip C. ; Smith, David Glenn. / Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta). In: American Journal of Primatology. 2006 ; Vol. 68, No. 1. pp. 73-95.
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