Microfluidic gel electrophoresis in the undergraduate laboratory applied to food analysis

Tzu Chiao Chao; Sanchari Bhattacharya; Alexandra Ros

doi:10.1021/ed101064p

Microfluidic gel electrophoresis in the undergraduate laboratory applied to food analysis

Tzu Chiao Chao, Sanchari Bhattacharya, Alexandra Ros

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are provided with berry samples from which they extract DNA and perform polymerase chain reaction (PCR) with strawberry-specific primers. The resulting PCR products are analyzed using the Agilent Bioanalyzer. Using the raw data, the students are tasked to identify the strawberry sample. This course serves as a practical introduction into microfluidic-based capillary gel electrophoresis as well as a primer for biomolecular DNA analysis.

Original language	English (US)
Pages (from-to)	125-129
Number of pages	5
Journal	Journal of Chemical Education
Volume	89
Issue number	1
DOIs	https://doi.org/10.1021/ed101064p
State	Published - Jan 1 2012

Keywords

Analytical chemistry
Bioanalytical chemistry
Electrophoresis
Food science
Hands-on learning/manipulatives
Laboratory instruction
Microscale lab
Nucleic acids/DNA/RNA
Separation science
Upper-division undergraduate

ASJC Scopus subject areas

General Chemistry
Education

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10.1021/ed101064p

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title = "Microfluidic gel electrophoresis in the undergraduate laboratory applied to food analysis",

abstract = "A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are provided with berry samples from which they extract DNA and perform polymerase chain reaction (PCR) with strawberry-specific primers. The resulting PCR products are analyzed using the Agilent Bioanalyzer. Using the raw data, the students are tasked to identify the strawberry sample. This course serves as a practical introduction into microfluidic-based capillary gel electrophoresis as well as a primer for biomolecular DNA analysis.",

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AU - Bhattacharya, Sanchari

AU - Ros, Alexandra

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N2 - A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are provided with berry samples from which they extract DNA and perform polymerase chain reaction (PCR) with strawberry-specific primers. The resulting PCR products are analyzed using the Agilent Bioanalyzer. Using the raw data, the students are tasked to identify the strawberry sample. This course serves as a practical introduction into microfluidic-based capillary gel electrophoresis as well as a primer for biomolecular DNA analysis.

AB - A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are provided with berry samples from which they extract DNA and perform polymerase chain reaction (PCR) with strawberry-specific primers. The resulting PCR products are analyzed using the Agilent Bioanalyzer. Using the raw data, the students are tasked to identify the strawberry sample. This course serves as a practical introduction into microfluidic-based capillary gel electrophoresis as well as a primer for biomolecular DNA analysis.

KW - Analytical chemistry

KW - Bioanalytical chemistry

KW - Electrophoresis

KW - Food science

KW - Hands-on learning/manipulatives

KW - Laboratory instruction

KW - Microscale lab

KW - Nucleic acids/DNA/RNA

KW - Separation science

KW - Upper-division undergraduate

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Microfluidic gel electrophoresis in the undergraduate laboratory applied to food analysis

Abstract

Keywords

ASJC Scopus subject areas

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