Microfluidic gel electrophoresis in the undergraduate laboratory applied to food analysis

Tzu Chiao Chao, Sanchari Bhattacharya, Alexandra Ros

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are provided with berry samples from which they extract DNA and perform polymerase chain reaction (PCR) with strawberry-specific primers. The resulting PCR products are analyzed using the Agilent Bioanalyzer. Using the raw data, the students are tasked to identify the strawberry sample. This course serves as a practical introduction into microfluidic-based capillary gel electrophoresis as well as a primer for biomolecular DNA analysis.

Original languageEnglish (US)
Pages (from-to)125-129
Number of pages5
JournalJournal of Chemical Education
Volume89
Issue number1
DOIs
StatePublished - Jan 1 2012

Keywords

  • Analytical chemistry
  • Bioanalytical chemistry
  • Electrophoresis
  • Food science
  • Hands-on learning/manipulatives
  • Laboratory instruction
  • Microscale lab
  • Nucleic acids/DNA/RNA
  • Separation science
  • Upper-division undergraduate

ASJC Scopus subject areas

  • Chemistry(all)
  • Education

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