Microbial gene expression elucidated by selective capture of transcribed sequences (SCOTS)

TY - JOUR

T1 - Microbial gene expression elucidated by selective capture of transcribed sequences (SCOTS)

AU - Daigle, France

AU - Hou, Joan Y.

AU - Clark-Curtiss, Josephine E.

N1 - Funding Information: We thank Eric Rubin for helpful comments on the manuscript and Sangita Chakraborty and Matthew Butler for troubleshooting and refining the transposase purification protocol. Application of the GAMBIT approach to H. influenzae and other pathogens was supported by a Horace H. Rackham Faculty Research Grant and the Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation Fellowship DRG-1371 to B.J.A. Work on Himarl transposase was supported by grants to D.J.L. from NIH-National Institute of Allergy and Infectious Diseases and NSF-Microbial Genetics. Funding Information: The authors acknowledge R. Curtiss III and C. M. Dozois for critical review of the manuscript. Methods and observations from the authors' laboratories arose during research supported by the National Institutes of Health (AI35267, AI38672, AI46428) to J.E.C.-C. and (AI24533) to R.C. HI and by a Canadian Natural Sciences and Engineering Research Council postdoctoral fellowship to ED.

PY - 2002

Y1 - 2002

UR - http://www.scopus.com/inward/record.url?scp=0036436719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036436719&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(02)58083-6

DO - 10.1016/S0076-6879(02)58083-6

M3 - Article

C2 - 12474381

AN - SCOPUS:0036436719

SN - 0076-6879

VL - 358

SP - 108

EP - 122

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -