Microbial diversity of benthic mats along a tidal desiccation gradient

Michael J. Rothrock, Ferran Garcia-Pichel

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

We investigated the influence of desiccation frequency, indicated by tidal position, on microbial community structure, diversity and richness of microbial mats. We independently characterized cyanobacterial, bacterial and archaeal communities, and their spatial variability for two distinct microbial mat systems: subtidal hypersaline mats and intertidal sand flat mats. Community fingerprints based on 16S rDNA were obtained via denaturing gradient gel electrophoresis using polymerase chain reaction primers specific for each group. Fingerprints for all three groups were consistently similar [≥85% according to Weighted Pair Group with Arithmetic Mean (WPGMA) analysis] along a 1-km-long transect in subtidal mats. Here, pair-wise comparison analysis yielded minimal variation in diversity and richness for all groups. Fingerprints of three sites along an intertidal transect were heterogenous (≥32% similarity according to WPGMA analysis) with clear shifts in community structure in all three microbial groups. Here, all groups exhibited statistically significant decreases in richness and diversity with tidal height (as desiccation frequency increases). Regression analysis yielded a strong correlation between diversity or richness estimates and position along the tidal gradient, for both Archaea and Bacteria, with Cyanobacteria exhibiting a weaker correlation.These results suggest that desiccation frequency can shape the structure of microbial mat communities, with Archea being least tolerant and Cyanobacteria most tolerant.

Original languageEnglish (US)
Pages (from-to)593-601
Number of pages9
JournalEnvironmental Microbiology
Volume7
Issue number4
DOIs
StatePublished - Apr 2005

Fingerprint

Desiccation
microbial mat
Dermatoglyphics
desiccation
Archaea
Cyanobacteria
cyanobacterium
community structure
transect
Denaturing Gradient Gel Electrophoresis
denaturing gradient gel electrophoresis
Ribosomal DNA
polymerase chain reaction
microbial communities
microbial community
electrokinesis
regression analysis
gel
Regression Analysis
sand

ASJC Scopus subject areas

  • Environmental Science(all)
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Microbial diversity of benthic mats along a tidal desiccation gradient. / Rothrock, Michael J.; Garcia-Pichel, Ferran.

In: Environmental Microbiology, Vol. 7, No. 4, 04.2005, p. 593-601.

Research output: Contribution to journalArticle

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