Methanolinea mesophila sp. nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov. within the order Methanomicrobiales

Sanae Sakai, Masayuki Ehara, I. Cheng Tseng, Takashi Yamaguchi, Suzanna L. Bräuer, Hinsby Cadillo-Quiroz, Stephen H. Zinder, Hiroyuki Imachi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A novel mesophilic, hydrogenotrophic methanogen, designated strain TNRT, was isolated from an anaerobic, propionate-degradation enrichment culture that was originally established from a rice field soil sample from Taiwan. Cells were non-motile rods, 2.0-6.5 μm long by 0.3 μm wide. Filamentous (up to about 100 μm) and coccoid (about 1 μm in diameter) cells were also observed in cultures in the late exponential phase of growth. Strain TNRT grew at 20-40°C (optimally at 37°C), at pH 6.5-7.4 (optimally at pH 7.0) and in the presence of 0-25 g NaCl l-1 (optimally at 0 g NaCl l-1). The strain utilized H2/CO2 and formate for growth and produced methane. The G+C content of the genomic DNA was 56.4 mol%. Based on sequences of both the 16S rRNA gene and the methanogen-specific marker gene mcrA, strain TNRT was related most closely to Methanolinea tarda NOBI-1T; levels of sequence similarities were 94.8 and 86.4%, respectively. The 16S rRNA gene sequence similarity indicates that strain TNRT and M. tarda NOBI-1T represent different species within the same genus. This is supported by shared phenotypic properties, including substrate usage and cell morphology, and differences in growth temperature. Based on these genetic and phenotypic properties, strain TNRT is considered to represent a novel species of the genus Methanolinea, for which the name Methanolinea mesophila sp. nov. is proposed; the type strain is TNRT (5NBRC 105659T5DSM 23604T). In addition, we also suggest family status for the E1/E2 group within the order Methanomicrobiales, for which the name Methanoregulaceae fam. nov. is proposed; the type genus of family is Methanoregula.

Original languageEnglish (US)
Pages (from-to)1389-1395
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume62
Issue number6
DOIs
StatePublished - Jun 2012

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Methanomicrobiales
methanogens
paddy field
agricultural soils
formic acid
Soil
rRNA Genes
Names
gene
new species
Growth
soil
Methane
Propionates
Base Composition
Taiwan
genomics
methane
DNA
substrate

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Methanolinea mesophila sp. nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov. within the order Methanomicrobiales. / Sakai, Sanae; Ehara, Masayuki; Tseng, I. Cheng; Yamaguchi, Takashi; Bräuer, Suzanna L.; Cadillo-Quiroz, Hinsby; Zinder, Stephen H.; Imachi, Hiroyuki.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 62, No. 6, 06.2012, p. 1389-1395.

Research output: Contribution to journalArticle

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abstract = "A novel mesophilic, hydrogenotrophic methanogen, designated strain TNRT, was isolated from an anaerobic, propionate-degradation enrichment culture that was originally established from a rice field soil sample from Taiwan. Cells were non-motile rods, 2.0-6.5 μm long by 0.3 μm wide. Filamentous (up to about 100 μm) and coccoid (about 1 μm in diameter) cells were also observed in cultures in the late exponential phase of growth. Strain TNRT grew at 20-40°C (optimally at 37°C), at pH 6.5-7.4 (optimally at pH 7.0) and in the presence of 0-25 g NaCl l-1 (optimally at 0 g NaCl l-1). The strain utilized H2/CO2 and formate for growth and produced methane. The G+C content of the genomic DNA was 56.4 mol{\%}. Based on sequences of both the 16S rRNA gene and the methanogen-specific marker gene mcrA, strain TNRT was related most closely to Methanolinea tarda NOBI-1T; levels of sequence similarities were 94.8 and 86.4{\%}, respectively. The 16S rRNA gene sequence similarity indicates that strain TNRT and M. tarda NOBI-1T represent different species within the same genus. This is supported by shared phenotypic properties, including substrate usage and cell morphology, and differences in growth temperature. Based on these genetic and phenotypic properties, strain TNRT is considered to represent a novel species of the genus Methanolinea, for which the name Methanolinea mesophila sp. nov. is proposed; the type strain is TNRT (5NBRC 105659T5DSM 23604T). In addition, we also suggest family status for the E1/E2 group within the order Methanomicrobiales, for which the name Methanoregulaceae fam. nov. is proposed; the type genus of family is Methanoregula.",
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AU - Ehara, Masayuki

AU - Tseng, I. Cheng

AU - Yamaguchi, Takashi

AU - Bräuer, Suzanna L.

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AU - Imachi, Hiroyuki

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