Abstract

A specially designed CH 4 -based membrane biofilm batch reactor (MBBR) was applied to investigate anaerobic methane oxidation coupled to perchlorate reduction (AnMO-PR). The 0.21 mM ClO 4 added in the first stage of operation was completely reduced in 28 days, 0.40 mM ClO 4 was reduced within 23 days in stage 2, and 0.56 mM of ClO 4 was reduced within 30 days in stage 3. Although some chlorate (ClO 3 ) accumulated, the recovery of Cl was over 92%. Illumina sequencing of the 16S rRNA gene documented that the bacterial community was mainly composed by perchlorate-reducing bacteria (PRB), methanotrophic bacteria, and archaea. Real-time quantitative PCR showed the archaeal 16S rRNA and mcrA genes increased as more ClO 4 was reduced, and the predominant archaea belonged to Methanosarcina mazei, which is related to ANME-3, an archaeon able to perform reverse methanogenesis. Several pieces of evidence support that ClO 4 reduction by the MBBR biofilm occurred via a synergism between Methanosarcina and PRB: Methanosarcina oxidized methane through reverse methanogesis and provided electron donor for PRB to reduce ClO 4 . Because methanotrophs were present, we cannot rule out that they also were involved in AnMO-PR if they received O 2 generated by disproportionation of ClO 2 from the PRB.

Original languageEnglish (US)
Pages (from-to)9-15
Number of pages7
JournalScience of the Total Environment
Volume667
DOIs
StatePublished - Jun 1 2019

Fingerprint

perchlorate
Methane
Batch reactors
Biofilms
biofilm
Bacteria
methane
membrane
Membranes
oxidation
Oxidation
bacterium
Genes
Chlorates
synergism
gene
methanogenesis
reactor
Recovery
electron

Keywords

  • Archaea
  • Bacteria
  • Membrane-biofilm batch reactor
  • Methane oxidation
  • Perchlorate reduction

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Methane oxidation coupled to perchlorate reduction in a membrane biofilm batch reactor. / Lv, Pan Long; Shi, Ling Dong; Wang, Zhen; Rittmann, Bruce; Zhao, He Ping.

In: Science of the Total Environment, Vol. 667, 01.06.2019, p. 9-15.

Research output: Contribution to journalArticle

Lv, Pan Long ; Shi, Ling Dong ; Wang, Zhen ; Rittmann, Bruce ; Zhao, He Ping. / Methane oxidation coupled to perchlorate reduction in a membrane biofilm batch reactor. In: Science of the Total Environment. 2019 ; Vol. 667. pp. 9-15.
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