Metabolomics Analysis of Viral Therapeutics

Haiwei Gu, Xiaojian Shi, Paniz Jasbi, Jeffrey Patterson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Virotherapy, enabled by recent advances in the transdisciplinary field of biotechnology, has emerged as a powerful tool for use in anticancer treatment, gene therapy, immunotherapy, etc. Examining the effects of viruses and virus-derived immune-modulating therapeutics is of great fundamental and clinical interest. Here we describe a sample preparation protocol for metabolite extraction from virus-infected tissue, in addition to liquid chromatography-mass spectrometry conditions essential for subsequent analysis. This metabolomics approach delivers highly sensitive and specific metabolite information on various biospecimens. Such an approach may be adopted to monitor biological changes in over 30 relevant metabolic pathways in response to viral infection and also viral therapeutics.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages179-197
Number of pages19
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2225
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • LC-MS/MS
  • Metabolomics
  • Sample preparation
  • Viral vector
  • Virotherapy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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