Membrane fusion during secretion. Cortical granule exocytosis in sea urchin eggs as studied by quick freezing and freeze-fracture

D. E. Chandler, J. Heuser

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Exocytosis of cortical granules was observed in sea urchin eggs, either quick-frozen or chemically fixed after exposure to sperm. Fertilization produced a wave of exocytosis that began within 20 s and swept across the egg surface in the following 30 s. The front of this wave was marked by fusion of single granules at well-separated sites. Toward the rear of the wave, granule fusion became so abundant that the egg surface was left with confluent patches of granule membrane. The resulting redundancy of the egg surface was accomodated by elaboration of characteristic branching microvilli, and by an intense burst of coated vesicle formation at ~ 2 min after insemination. Freeze-fracture replicas of eggs fixed with glutaraldehyde and soaked in glycerol before freezing displayed forms of granule membrane interaction with the plasma membrane which looked like what other investigators have considered to be intermediates in exocytosis. These were small disks of membrane contact or membrane fusion, which often occurred in multiple sites on one granule and also between adjacent granules. However, such membrane interactions were never found in eggs that were quick-frozen without fixation, or in eggs fixed and frozen without exposure to glycerol. Glycerination of fixed material appeared to be the important variable; more concentrated glycerol produced a greater abundance of such 'intermediates'. Thus, these structures may be artifacts produced by dehydrating chemically fixed membranes, and may not be directly relevant to the mechanism by which membranes naturally fuse.

Original languageEnglish (US)
Pages (from-to)91-108
Number of pages18
JournalJournal of Cell Biology
Volume83
Issue number1
StatePublished - 1979
Externally publishedYes

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Membrane Fusion
Sea Urchins
Exocytosis
Eggs
Freezing
Membranes
Glycerol
Ovum
Coated Vesicles
Insemination
Glutaral
Microvilli
Fertilization
Artifacts
Spermatozoa
Research Personnel
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

Membrane fusion during secretion. Cortical granule exocytosis in sea urchin eggs as studied by quick freezing and freeze-fracture. / Chandler, D. E.; Heuser, J.

In: Journal of Cell Biology, Vol. 83, No. 1, 1979, p. 91-108.

Research output: Contribution to journalArticle

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