Mechanical and physical properties of recombinant spider silk films using organic and aqueous solvents

Chauncey L. Tucker, Justin A. Jones, Heidi N. Bringhurst, Cameron G. Copeland, J. Bennett Addison, Warner S. Weber, Qiushi Mou, Jeffery Yarger, Randolph V. Lewis

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Spider silk has exceptional mechanical and biocompatibility properties. The goal of this study was optimization of the mechanical properties of synthetic spider silk thin films made from synthetic forms of MaSp1 and MaSp2, which compose the dragline silk of Nephila clavipes. We increased the mechanical stress of MaSp1 and 2 films solubilized in both HFIP and water by adding glutaraldehyde and then stretching them in an alcohol based stretch bath. This resulted in stresses as high as 206 MPa and elongations up to 35%, which is 4× higher than the as-poured controls. Films were analyzed using NMR, XRD, and Raman, which showed that the secondary structure after solubilization and film formation in as-poured films is mainly a helical conformation. After the post-pour stretch in a methanol/water bath, the MaSp proteins in both the HFIP and water-based films formed alignedβ-sheets similar to those in spider silk fibers.

Original languageEnglish (US)
Pages (from-to)3158-3170
Number of pages13
JournalBiomacromolecules
Volume15
Issue number8
DOIs
StatePublished - Aug 11 2014

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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