Measurement of Eicosanoids in Cancer Tissues

Dingzhi Wang, Raymond N. DuBois

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Eicosanoids derived from arachidonic acid through cyclooxygenase (COX), lipoxygenase (LOX), and P450 pathways include prostanoids, hydroxyeicosatetraenoic acids (HETEs), leukotrienes (LTs), and epoxyeicosatrienoic acids (EETs). These bioactive lipids play an important role in regulating cell proliferation, apoptosis, tissue repair, blood clotting, blood vessel permeability, inflammation, and immune cell behavior. Moreover, some of these eicosanoids also modulate inflammation and tumor growth in cancer tissues, and may serve as biomarkers for monitoring colorectal cancer progression or as an intermediate marker for the pharmacologic activity of chemopreventive agents. Development of sensitive, rapid, and specific methods for determining eicosanoid levels accurately will facilitate an understanding of the biologic functions of these lipid mediators and will broaden our insight of the importance of these bioactive lipids in vivo. However, quantitative determination of eicosanoids in biological samples has presented a problem to many investigators. It is necessary to understand the advantages and limitations of each method for quantitative analysis of specific eicosanoids in various types of biological samples. Here we evaluate the methodology of the measurement of eicosanoids in biological samples.

Original languageEnglish (US)
Pages (from-to)27-50
Number of pages24
JournalMethods in Enzymology
Volume433
DOIs
StatePublished - 2007
Externally publishedYes

Fingerprint

Eicosanoids
Tissue
Hydroxyeicosatetraenoic Acids
Neoplasms
Lipids
Inflammation
Lipoxygenase
Leukotrienes
Blood vessels
Cell proliferation
Blood Coagulation
Biomarkers
Prostaglandin-Endoperoxide Synthases
Arachidonic Acid
Prostaglandins
Blood Vessels
Tumors
Colorectal Neoplasms
Permeability
Repair

ASJC Scopus subject areas

  • Biochemistry

Cite this

Measurement of Eicosanoids in Cancer Tissues. / Wang, Dingzhi; DuBois, Raymond N.

In: Methods in Enzymology, Vol. 433, 2007, p. 27-50.

Research output: Contribution to journalArticle

Wang, Dingzhi ; DuBois, Raymond N. / Measurement of Eicosanoids in Cancer Tissues. In: Methods in Enzymology. 2007 ; Vol. 433. pp. 27-50.
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