Maximizing the taxonomic resolution of MALDI-TOF-MS-based approaches to bacterial characterization: From culture conditions through data analysis

Lin Zhang, Todd Sandrin

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be a rapid, effective, and sensitive tool for characterizing diverse bacteria. Historically, the primary application of this technology has been to identify bacteria at the genus and species levels in a few minutes. The most commonly used identification method involves matching mass spectra of unknown bacteria to reference spectra in commercially available databases with the assistance of manufacturer-created algorithms. Recently, there has been keen interest in enhancing the taxonomic resolution of MALDI to characterize bacteria at the strain and subspecies levels. Often, categorization of strains sharing similar traits, differentiation of closely related strains, and/or identification of a single strain are desired. Strain-level characterization is important in the development of counter-bioterrorism strategies, prevention of food contamination, and rapid diagnosis of bacterial infections. This chapter focuses on factors, including culture medium, sample preparation, data acquisition, and data analysis that affect strain-level characterization. In addition, the chapter highlights techniques and treatments that offer promise to improve the taxonomic resolution of MALDI-TOF MS-enabled approaches to bacterial characterization.

Original languageEnglish (US)
Title of host publicationApplications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance
PublisherSpringer International Publishing
Pages147-181
Number of pages35
ISBN (Print)9783319260709, 9783319260686
DOIs
StatePublished - Jan 1 2016

Keywords

  • Culture conditions
  • Data acquisition
  • Data analysis
  • MALDI-TOF MS
  • Optimization
  • Reproducibility
  • Sample preparation
  • Strain
  • Taxonomic resolution

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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    Zhang, L., & Sandrin, T. (2016). Maximizing the taxonomic resolution of MALDI-TOF-MS-based approaches to bacterial characterization: From culture conditions through data analysis. In Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance (pp. 147-181). Springer International Publishing. https://doi.org/10.1007/978-3-319-26070-9_6