Mass spectra of ribonucleoside components of tRNA. II

Sidney Hecht, Amrit S. Gupta, Nelson J. Leonard

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have obtained low-resolution mass spectra under as uniform conditions as possible at 20 and 70 eV for the following ribonucleosides: cytidine, uridine, adenosine, 5-hydroxymethyluridine, 2-methyladenosine, 2′-O-methyladenosine, isoguanosine, xanthosine, 2′-O-methylguanosine, N2,N2-dimethylguanosine, N6-(3-methyl-2-butenyl)adenosine, N6-(3-methyl-3-butenyl)adenosine, ribosylzeatin, N6-(3-hydroxy-3-methyl-butyl)adenosine, ribosyldihydrozeatin, and N6-(3-methyl-2-butenyl)-2-methylthioadenosine. All these ribonucleosides are components, or possible components, of tRNA. The spectra are intended as reference spectra for the structural confirmation of known nucleosides from tRNA and for the identification of unknown, modified nucleosides by comparison of their fragmentation pattern with those already recorded.

Original languageEnglish (US)
Pages (from-to)230-251
Number of pages22
JournalAnalytical Biochemistry
Volume38
Issue number1
DOIs
StatePublished - 1970
Externally publishedYes

Fingerprint

Ribonucleosides
Transfer RNA
Adenosine
Nucleosides
Cytidine
Uridine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Mass spectra of ribonucleoside components of tRNA. II. / Hecht, Sidney; Gupta, Amrit S.; Leonard, Nelson J.

In: Analytical Biochemistry, Vol. 38, No. 1, 1970, p. 230-251.

Research output: Contribution to journalArticle

Hecht, Sidney ; Gupta, Amrit S. ; Leonard, Nelson J. / Mass spectra of ribonucleoside components of tRNA. II. In: Analytical Biochemistry. 1970 ; Vol. 38, No. 1. pp. 230-251.
@article{4775734d56f241f1b026671445e2d207,
title = "Mass spectra of ribonucleoside components of tRNA. II",
abstract = "We have obtained low-resolution mass spectra under as uniform conditions as possible at 20 and 70 eV for the following ribonucleosides: cytidine, uridine, adenosine, 5-hydroxymethyluridine, 2-methyladenosine, 2′-O-methyladenosine, isoguanosine, xanthosine, 2′-O-methylguanosine, N2,N2-dimethylguanosine, N6-(3-methyl-2-butenyl)adenosine, N6-(3-methyl-3-butenyl)adenosine, ribosylzeatin, N6-(3-hydroxy-3-methyl-butyl)adenosine, ribosyldihydrozeatin, and N6-(3-methyl-2-butenyl)-2-methylthioadenosine. All these ribonucleosides are components, or possible components, of tRNA. The spectra are intended as reference spectra for the structural confirmation of known nucleosides from tRNA and for the identification of unknown, modified nucleosides by comparison of their fragmentation pattern with those already recorded.",
author = "Sidney Hecht and Gupta, {Amrit S.} and Leonard, {Nelson J.}",
year = "1970",
doi = "10.1016/0003-2697(70)90173-9",
language = "English (US)",
volume = "38",
pages = "230--251",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Mass spectra of ribonucleoside components of tRNA. II

AU - Hecht, Sidney

AU - Gupta, Amrit S.

AU - Leonard, Nelson J.

PY - 1970

Y1 - 1970

N2 - We have obtained low-resolution mass spectra under as uniform conditions as possible at 20 and 70 eV for the following ribonucleosides: cytidine, uridine, adenosine, 5-hydroxymethyluridine, 2-methyladenosine, 2′-O-methyladenosine, isoguanosine, xanthosine, 2′-O-methylguanosine, N2,N2-dimethylguanosine, N6-(3-methyl-2-butenyl)adenosine, N6-(3-methyl-3-butenyl)adenosine, ribosylzeatin, N6-(3-hydroxy-3-methyl-butyl)adenosine, ribosyldihydrozeatin, and N6-(3-methyl-2-butenyl)-2-methylthioadenosine. All these ribonucleosides are components, or possible components, of tRNA. The spectra are intended as reference spectra for the structural confirmation of known nucleosides from tRNA and for the identification of unknown, modified nucleosides by comparison of their fragmentation pattern with those already recorded.

AB - We have obtained low-resolution mass spectra under as uniform conditions as possible at 20 and 70 eV for the following ribonucleosides: cytidine, uridine, adenosine, 5-hydroxymethyluridine, 2-methyladenosine, 2′-O-methyladenosine, isoguanosine, xanthosine, 2′-O-methylguanosine, N2,N2-dimethylguanosine, N6-(3-methyl-2-butenyl)adenosine, N6-(3-methyl-3-butenyl)adenosine, ribosylzeatin, N6-(3-hydroxy-3-methyl-butyl)adenosine, ribosyldihydrozeatin, and N6-(3-methyl-2-butenyl)-2-methylthioadenosine. All these ribonucleosides are components, or possible components, of tRNA. The spectra are intended as reference spectra for the structural confirmation of known nucleosides from tRNA and for the identification of unknown, modified nucleosides by comparison of their fragmentation pattern with those already recorded.

UR - http://www.scopus.com/inward/record.url?scp=0014870712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014870712&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(70)90173-9

DO - 10.1016/0003-2697(70)90173-9

M3 - Article

VL - 38

SP - 230

EP - 251

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -