Mass spectra of nucleoside components of tRNA

Sidney Hecht, Amrit S. Gupta, Nelson J. Leonard

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Low resolution mass spectra have been recorded at 20 eV and 70 eV under comparable conditions for a series of nucleosides which are components, or possible components, of tRNA: pseudouridine, 5,6-dihydrouridine, 5-methylcytidine, ribothymidine, 3-methyluridine, inosine, 1-methyladenosine, N6-methyladenosine, 1-methylinosine, guanosine, N4-acetylcytidine, N6, N6-dimethyladenosine, N2-methylguanosine, 1-methylguanosine, 7-methylguanosine, and 2-methoxyadenosine. These spectra can be used for the identification of presently known nucleosides and for the identification of newly isolated ribosides by consideration of their fragmentation patterns relative to those of the known compounds.

Original languageEnglish (US)
Pages (from-to)249-270
Number of pages22
JournalAnalytical Biochemistry
Volume30
Issue number2
StatePublished - Aug 1969
Externally publishedYes

Fingerprint

Transfer RNA
Nucleosides
Pseudouridine
Inosine
Guanosine
8-methylguanosine
5-methylcytidine
7-methylguanosine
3-methyluridine
ribothymidine
N-acetylcytidine
5,6-dihydrouridine
1-methyladenosine
1-methylinosine
1-methylguanosine

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Hecht, S., Gupta, A. S., & Leonard, N. J. (1969). Mass spectra of nucleoside components of tRNA. Analytical Biochemistry, 30(2), 249-270.

Mass spectra of nucleoside components of tRNA. / Hecht, Sidney; Gupta, Amrit S.; Leonard, Nelson J.

In: Analytical Biochemistry, Vol. 30, No. 2, 08.1969, p. 249-270.

Research output: Contribution to journalArticle

Hecht, S, Gupta, AS & Leonard, NJ 1969, 'Mass spectra of nucleoside components of tRNA', Analytical Biochemistry, vol. 30, no. 2, pp. 249-270.
Hecht S, Gupta AS, Leonard NJ. Mass spectra of nucleoside components of tRNA. Analytical Biochemistry. 1969 Aug;30(2):249-270.
Hecht, Sidney ; Gupta, Amrit S. ; Leonard, Nelson J. / Mass spectra of nucleoside components of tRNA. In: Analytical Biochemistry. 1969 ; Vol. 30, No. 2. pp. 249-270.
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