TY - JOUR
T1 - Mass spectra of nucleoside components of tRNA
AU - Hecht, Sidney M.
AU - Gupta, Amrit S.
AU - Leonard, Nelson J.
N1 - Funding Information:
This work was supported by a research grant (GM-05829) from the National Institutes of Health, U. S. Public Health Service, and by a National Institutes Health Predoctoral Fellowship to one of us (S.M.H.), 1967-1969. We are indebted to Mr. J. Wrona determining the mass spect.ra.
PY - 1969/8
Y1 - 1969/8
N2 - Low resolution mass spectra have been recorded at 20 eV and 70 eV under comparable conditions for a series of nucleosides which are components, or possible components, of tRNA: pseudouridine, 5,6-dihydrouridine, 5-methylcytidine, ribothymidine, 3-methyluridine, inosine, 1-methyladenosine, N6-methyladenosine, 1-methylinosine, guanosine, N4-acetylcytidine, N6, N6-dimethyladenosine, N2-methylguanosine, 1-methylguanosine, 7-methylguanosine, and 2-methoxyadenosine. These spectra can be used for the identification of presently known nucleosides and for the identification of newly isolated ribosides by consideration of their fragmentation patterns relative to those of the known compounds.
AB - Low resolution mass spectra have been recorded at 20 eV and 70 eV under comparable conditions for a series of nucleosides which are components, or possible components, of tRNA: pseudouridine, 5,6-dihydrouridine, 5-methylcytidine, ribothymidine, 3-methyluridine, inosine, 1-methyladenosine, N6-methyladenosine, 1-methylinosine, guanosine, N4-acetylcytidine, N6, N6-dimethyladenosine, N2-methylguanosine, 1-methylguanosine, 7-methylguanosine, and 2-methoxyadenosine. These spectra can be used for the identification of presently known nucleosides and for the identification of newly isolated ribosides by consideration of their fragmentation patterns relative to those of the known compounds.
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U2 - 10.1016/0003-2697(69)90396-0
DO - 10.1016/0003-2697(69)90396-0
M3 - Article
C2 - 5809667
AN - SCOPUS:0014558894
SN - 0003-2697
VL - 30
SP - 249
EP - 270
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -