TY - JOUR
T1 - Low temperature EPR on photosystem I single crystals
T2 - Orientation of the iron-sulfur centers F(A) and F(B)
AU - Kamlowski, Andreas
AU - Van Der Est, Arthur
AU - Fromme, Petra
AU - Stehlik, Dietmar
N1 - Funding Information:
We would like to acknowledge Dr. N. Krauß, W.-D. Schubert (FU Berlin, Germany) and Prof. H.T. Witt (TU Berlin, Germany) for discussion and cooperation. We also thank Drs. C. Ross (University of Nebraska, USA) and B. Lamotte (C.E.A. Grenoble, France) for helpful discussions in the development of the evaluation strategy. We acknowledge Prof. J. Hüttermann (Univ. des Saarlandes, Germany) for sending us a preprint. This work was supported by the Deutsche Forschungsgemeinschaft (SfB 312; Teilprojekte A1 und A3).
PY - 1997/4/11
Y1 - 1997/4/11
N2 - Low temperature EPR results from Photosystem I (PS I) single crystals of Synechococcus elongatus are presented. Illumination at 150 K and pH 6.4 is used to photoreduce the two terminal 4Fe-4S centers to the noninteracting state (F(A)-) + (F(B)-). From the EPR data and the analysis of the rotation pattern for both F(A)- and F(B)- the following information is obtained: (i) the principal values of the g tensors of F(A)- and F(B)-, (ii) the orientations of their principal g tensor axes with respect to the crystal axes for each of the six PS I centers per unit cell, and (iii) their orientation with respect to one another. In addition, significant differences between F(A)- and F(B)- are noted with respect to the orientational dependence of the linewidth and the saturation behavior of their EPR signals. In principle, six relative arrangements of the g tensors of F(A)- and F(B)- are consistent with the EPR data. Only two out of these six are compatible with the known structure of the bacterial fenedoxin from P. aerogenes (PaFd) (Adman, E.T., Siecker, L.C, and Jensen, L.H. (1976) J. Biol. Chem. 251, 3801) which has been used as a model for the core of the PsaC protein carrying F(A) and F(B) in PS I. It is concluded that the PaFd and the PsaC protein are analogous with respect to the central part of their structures. The results and conclusions are compared to those obtained from studies on oriented membranes.
AB - Low temperature EPR results from Photosystem I (PS I) single crystals of Synechococcus elongatus are presented. Illumination at 150 K and pH 6.4 is used to photoreduce the two terminal 4Fe-4S centers to the noninteracting state (F(A)-) + (F(B)-). From the EPR data and the analysis of the rotation pattern for both F(A)- and F(B)- the following information is obtained: (i) the principal values of the g tensors of F(A)- and F(B)-, (ii) the orientations of their principal g tensor axes with respect to the crystal axes for each of the six PS I centers per unit cell, and (iii) their orientation with respect to one another. In addition, significant differences between F(A)- and F(B)- are noted with respect to the orientational dependence of the linewidth and the saturation behavior of their EPR signals. In principle, six relative arrangements of the g tensors of F(A)- and F(B)- are consistent with the EPR data. Only two out of these six are compatible with the known structure of the bacterial fenedoxin from P. aerogenes (PaFd) (Adman, E.T., Siecker, L.C, and Jensen, L.H. (1976) J. Biol. Chem. 251, 3801) which has been used as a model for the core of the PsaC protein carrying F(A) and F(B) in PS I. It is concluded that the PaFd and the PsaC protein are analogous with respect to the central part of their structures. The results and conclusions are compared to those obtained from studies on oriented membranes.
KW - EPR
KW - F(A)
KW - F(B)
KW - Iron-sulfur center
KW - Low temperature EPR
KW - Photoreduction
KW - Photosystem I crystal
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U2 - 10.1016/S0005-2728(96)00161-2
DO - 10.1016/S0005-2728(96)00161-2
M3 - Article
AN - SCOPUS:0030980887
SN - 0005-2728
VL - 1319
SP - 185
EP - 198
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 2-3
ER -