Long-range heterogeneity at the 3′ ends of human mRNAs

Christian Iseli, Brian J. Stevenson, Sandro J. De Souza, Helena B. Samaia, Anamaria A. Camargo, Kenneth Buetow, Robert L. Strausberg, Andrew J G Simpson, Philipp Bucher, C. Victor Jongeneel

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The publication of a draft of the human genome and of large collections of transcribed sequences has made it possible to study the complex relationship between the transcriptome and the genome. In the work presented here, we have focused on mapping mRNA 3′ ends onto the genome by use of the raw data generated by the expressed sequence tag (EST) sequencing projects. We find that at least half of the human genes encode multiple transcripts whose polyadenylation is driven by multiple signals. The corresponding transcript 3′ ends are spread over distances in the kilobase range. This finding has profound implications for our understanding of gene expression regulation and of the diversity of human transcripts, for the design of cDNA microarray probes, and for the interpretation of gene expression profiling experiments.

Original languageEnglish (US)
Pages (from-to)1068-1074
Number of pages7
JournalGenome Research
Volume12
Issue number7
DOIs
StatePublished - 2002
Externally publishedYes

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Genome
Messenger RNA
Polyadenylation
Expressed Sequence Tags
Gene Expression Regulation
Gene Expression Profiling
Human Genome
Oligonucleotide Array Sequence Analysis
Transcriptome
Publications
Complementary DNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Iseli, C., Stevenson, B. J., De Souza, S. J., Samaia, H. B., Camargo, A. A., Buetow, K., ... Victor Jongeneel, C. (2002). Long-range heterogeneity at the 3′ ends of human mRNAs. Genome Research, 12(7), 1068-1074. https://doi.org/10.1101/gr.62002. Article published online before print in June 2002

Long-range heterogeneity at the 3′ ends of human mRNAs. / Iseli, Christian; Stevenson, Brian J.; De Souza, Sandro J.; Samaia, Helena B.; Camargo, Anamaria A.; Buetow, Kenneth; Strausberg, Robert L.; Simpson, Andrew J G; Bucher, Philipp; Victor Jongeneel, C.

In: Genome Research, Vol. 12, No. 7, 2002, p. 1068-1074.

Research output: Contribution to journalArticle

Iseli, C, Stevenson, BJ, De Souza, SJ, Samaia, HB, Camargo, AA, Buetow, K, Strausberg, RL, Simpson, AJG, Bucher, P & Victor Jongeneel, C 2002, 'Long-range heterogeneity at the 3′ ends of human mRNAs', Genome Research, vol. 12, no. 7, pp. 1068-1074. https://doi.org/10.1101/gr.62002. Article published online before print in June 2002
Iseli, Christian ; Stevenson, Brian J. ; De Souza, Sandro J. ; Samaia, Helena B. ; Camargo, Anamaria A. ; Buetow, Kenneth ; Strausberg, Robert L. ; Simpson, Andrew J G ; Bucher, Philipp ; Victor Jongeneel, C. / Long-range heterogeneity at the 3′ ends of human mRNAs. In: Genome Research. 2002 ; Vol. 12, No. 7. pp. 1068-1074.
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