Localized protein synthesis during oogenesis of Xenopus laevis: Analysis by in situ translation

David G. Capco; Herbert Jäckle

doi:10.1016/0012-1606(82)90066-5

Localized protein synthesis during oogenesis of Xenopus laevis: Analysis by in situ translation

David G. Capco, Herbert Jäckle

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

An in situ translation assay was developed to examine localized protein synthesis activity on the subcellular level of fixed and sectioned material by autoradiography. Our data indicate that in situ translation produced polypeptides also synthesized in vivo and is dependent on protein synthesis from messenger RNA preserved in the fixed material. Applied to oocytes of Xenopus laevis at different stages, the in situ translation assay revealed localized protein synthesis activity in the cortical region of vitellogenic and postvitellogenic oocytes. The spatial pattern of protein synthesis activity observed in stage 6 oocytes disappeared when oocytes were induced to mature.

Original language	English (US)
Pages (from-to)	41-50
Number of pages	10
Journal	Developmental Biology
Volume	94
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(82)90066-5
State	Published - Nov 1982
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

Access to Document

10.1016/0012-1606(82)90066-5

Cite this

@article{df1aa86184354440b626077cf86d5683,

title = "Localized protein synthesis during oogenesis of Xenopus laevis: Analysis by in situ translation",

abstract = "An in situ translation assay was developed to examine localized protein synthesis activity on the subcellular level of fixed and sectioned material by autoradiography. Our data indicate that in situ translation produced polypeptides also synthesized in vivo and is dependent on protein synthesis from messenger RNA preserved in the fixed material. Applied to oocytes of Xenopus laevis at different stages, the in situ translation assay revealed localized protein synthesis activity in the cortical region of vitellogenic and postvitellogenic oocytes. The spatial pattern of protein synthesis activity observed in stage 6 oocytes disappeared when oocytes were induced to mature.",

author = "Capco, {David G.} and Herbert J{\"a}ckle",

year = "1982",

month = nov,

doi = "10.1016/0012-1606(82)90066-5",

language = "English (US)",

volume = "94",

pages = "41--50",

journal = "Developmental Biology",

issn = "0012-1606",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Localized protein synthesis during oogenesis of Xenopus laevis

T2 - Analysis by in situ translation

AU - Capco, David G.

AU - Jäckle, Herbert

PY - 1982/11

Y1 - 1982/11

N2 - An in situ translation assay was developed to examine localized protein synthesis activity on the subcellular level of fixed and sectioned material by autoradiography. Our data indicate that in situ translation produced polypeptides also synthesized in vivo and is dependent on protein synthesis from messenger RNA preserved in the fixed material. Applied to oocytes of Xenopus laevis at different stages, the in situ translation assay revealed localized protein synthesis activity in the cortical region of vitellogenic and postvitellogenic oocytes. The spatial pattern of protein synthesis activity observed in stage 6 oocytes disappeared when oocytes were induced to mature.

AB - An in situ translation assay was developed to examine localized protein synthesis activity on the subcellular level of fixed and sectioned material by autoradiography. Our data indicate that in situ translation produced polypeptides also synthesized in vivo and is dependent on protein synthesis from messenger RNA preserved in the fixed material. Applied to oocytes of Xenopus laevis at different stages, the in situ translation assay revealed localized protein synthesis activity in the cortical region of vitellogenic and postvitellogenic oocytes. The spatial pattern of protein synthesis activity observed in stage 6 oocytes disappeared when oocytes were induced to mature.

UR - http://www.scopus.com/inward/record.url?scp=0020446307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020446307&partnerID=8YFLogxK

U2 - 10.1016/0012-1606(82)90066-5

DO - 10.1016/0012-1606(82)90066-5

M3 - Article

C2 - 6130012

AN - SCOPUS:0020446307

SN - 0012-1606

VL - 94

SP - 41

EP - 50

JO - Developmental Biology

JF - Developmental Biology

IS - 1

ER -

Localized protein synthesis during oogenesis of Xenopus laevis: Analysis by in situ translation

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this